酸枣仁皂苷A对缺血再灌注损伤大鼠心律失常及Bcl-2、Bax表达的影响  

Effects of Jujuboside A on Arrhythmia and Expression of Bcl-2 and Bax in Rats with Myocardial Ischemia Reperfusion Injury

作　　者：黄宜生[1] 贾钰华[1] 孙学刚[1] 潘芸芸[1] 

机构地区：[1]南方医科大学中医药学院,广州510515

出　　处：《中药新药与临床药理》2011年第1期51-54,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金课题(30572435)

摘　　要：目的研究酸枣仁皂苷A对缺血再灌注损伤大鼠心律失常、心肌组织的保护及Bcl-2和Bax表达的影响。方法将24只Wistar雄性大鼠随机分为假手术组、模型组和酸枣仁皂苷A组(20 mg.kg～1.d～1),采用结扎(15 min)和松开(30 min)左冠状动脉前降支的方法制备心肌缺血再灌注损伤模型。记录再灌注过程中II导联心电图,并对心律失常进行评分;常规HE染色观察心肌组织的病理改变;免疫印迹技术检测心肌组织中Bcl-2、Bax的表达。结果模型组心肌组织病理损伤明显,有明显的心律失常,心肌Bcl-2表达显著降低,Bax表达显著升高。酸枣仁皂苷A干预后,心肌组织病理改变减轻,心律失常评分显著降低,心肌组织Bcl-2表达显著升高,Bax表达显著降低,与模型组比较有显著性差异(P<0.05)。结论酸枣仁皂苷A对缺血再灌注损伤大鼠心律失常具有保护作用,可抑制再灌注损伤后大鼠心肌组织Bcl-2降低和Bax表达的升高。Objective To study the effect of jujuboside A with myocardial ischemia reperfusion injury. Methods on arrhythmia and expression of Bcl-2 and Bax in rats Twenty-four male rats were randomly divided into three groups： sham-operation group, model group and jujuboside A group （in the dose of 20 mg·kg^-1·d^-1）. The animal model of myocardial ischemia reperfusion injury were established by constricting the left coronary arteries for 15 min, and then loosing for 30 min. Arrhythmia was scored according to 2-lead electrocardiogram. HE staining method was used to observe the pathological changes of myoeatdium. The expression of Bel-2 and Bax in myocardium was detected by western blotting. Results Isehemic injury changes of the myocardium and obvious arrhythmia occurred, myocardial Bcl-2 expression decreased and Bax expression increased significantly in the model group. Compared with model group, jujuboside A relieved the pathology changes in myoeardium, decreased arrhythmia score and Bax level significantly, and increased the expression of Bcl-2 （P 〈 0.05）. Conclusion Jujuboside A has a protective effect against arrhythmia of myocardial isehemia repeffusion injury in rats, which can inhibit the decrease of Bcl-2 and the increase of Bax after the repeffusion injury.

关 键 词：酸枣仁皂苷A 缺血再灌注损伤 心律失常 BCL-2 BAX 大鼠 

分 类 号：R285.5[医药卫生—中药学]