黄芪和白术提取物对IEC-6细胞迁移的影响  

Effects of Astragalus and Atractylodes Extracts on IEC-6 Cell Migration

作　　者：胡灿[1] 李茹柳[1] 王静[1] 徐颂芬[1] 陈蔚文[1,2] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]上海市高校中医内科学E-研究院,上海中医药大学,上海201203

出　　处：《中药新药与临床药理》2011年第1期60-65,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(30772753);广东省自然科学基金项目(7300343);上海市教育委员会E-研究院建设计划项目(E03008)

摘　　要：目的筛选益气健脾中药黄芪和白术促进小肠上皮细胞(IEC-6)迁移的有效提取部位。方法以系统溶剂分离法从黄芪、白术药材分别得到糖复合物、正丁醇萃取物、醇沉上清液以及大孔树脂吸附后70%乙醇洗脱部位作受试药。IEC-6细胞接种于6孔板并培养24 h,划痕法造细胞迁移模型,划痕后加入各受试药,24 h后观察细胞迁移情况;并进一步观察具有促进细胞迁移的有效部位对二氟甲基鸟氨酸(DFMO)所致细胞迁移抑制的影响。结果 50 mg.L-1的黄芪糖复合物或白术糖复合物能促进细胞迁移(P<0.01);黄芪、白术的正丁醇萃取物、醇沉上清液、大孔树脂吸附后醇洗脱部位对细胞迁移均无明显影响;2.5 mmol.L-1的DFMO能抑制细胞迁移(P<0.01),加入DFMO同时加入100 mg.L-1黄芪糖复合物或200 mg.L-1白术糖复合物能使细胞迁移恢复至接近正常水平。结论黄芪糖复合物和白术糖复合物能促进IEC-6细胞迁移,还能使DFMO所致的细胞迁移抑制恢复至接近正常水平。Objective To screen the effective fraction of Radix Astragali and Rhizoma Atractylodis Macrocephalae, which have the actions of strengthening spleen and replenishing Qi, for promoting intestine epithelial cell 6 （IEC-6）migration. Methods The testing agents included polysaccharides, N-butanol extracts and supernatant of ethanol precipitation from Radix Astragali and Rhizoma Atractylodis Macrocephalae prepared by systematic solvent extraction firstly, and then with 70 % ethanol eluent after D-101 macroporous resin adsorption. Cells were implanted in 6-well plates. Cell migration model was induced by scratch method in each well after cuhuring for 24 h, and then the CDMEN contained testing agents was added in. Twenty-four hours later, the migrating cells were counted to screen the effective fraction for promoting cell migration. The effects of the extracts on promoting cell migration were observed by giving a migration inhibiter of -difluoromethylornithine （DFMO）. Results Polysaccharides from Radix Astragali（50 mg·L^-1）and Rhizoma Atractylodis Macrocephalae（50 mg·L^-1） both promoted IEC-6 cell migration（P 〈 0.01 compared with the blank group）. N-butanol extract, ethanol supernatant prepared by systematic solvent extraction, and 70% ethanol eluent prepared by D-101 macroporous resin adsorption from Radix Astragali and Rhizoma Atractylodis Macrocephalae had no significant affect on the cell migration. DFMO （2.5 mmol·L^-1）inhibited the cell migration（P〈 0.01 compared with the blank group）. The polysaccharides from Radix Astragali（ 100 mg·L^-1）and Rhizoma Atractylodis Macrocephalae（200 mg·L^-1）reversed the DFMO-induced cell migration inhibition to normal level（P 〈 0.01and P 〈 0.05 compared with the model group; P〉 0.05 compared with the blank group）. Conclusion The polysaccharides from Radix Astragali and Rhizoma Atractylodis Macrocephalae p can promote IEC-6 cell migration, and reverse the DFMO-induced cell migration inhibition to normal levels.

关 键 词：小肠上皮细胞 黄芪 白术 提取物 细胞迁移 

分 类 号：R285.5[医药卫生—中药学]