加味七方胃痛颗粒对慢性萎缩性胃炎大鼠胃蛋白酶含量及HSP70蛋白表达的影响  

Effect of Modified Qifang Weitong Granules on Pepsin Content and Heat Shock Protein 70 Expression in Rats with Chronic Atrophic Gastritis

作　　者：唐梅文[1] 王伟[1] 李桂贤[2] 岳小红[1] 陈国忠[2] 邓嫦[2] 

机构地区：[1]广西中医学院,南宁530001 [2]广西中医学院第一附属医院,南宁530023

出　　处：《中药新药与临床药理》2011年第1期69-72,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广西自然科学基金项目(桂科自0991156)

摘　　要：目的探讨加味七方胃痛颗粒对慢性萎缩性胃炎(CAG)模型大鼠胃黏膜组织中胃蛋白酶含量及热休克蛋白70(HSP70)表达的影响。方法采用化学致癌物N-甲基-N謘-硝基-N-亚硝基胍(MNNG)建立CAG动物模型,以胃复春为对照组,观察加味七方胃痛颗粒对大鼠CAG相关指标的作用。结果与模型组比较,加味七方胃痛颗粒高、中剂量组和胃复春组的胃黏膜萎缩程度、胃黏膜肠化生程度明显减轻(P<0.01);加味七方胃痛颗粒高、中、低剂量组和胃复春组胃蛋白酶的含量、HSP70蛋白表达显著增加(P<0.01);与胃复春组比较,加味七方胃痛颗粒高剂量组胃蛋白酶的含量、HSP70蛋白表达显著增加(P<0.05)。结论加味七方胃痛颗粒防治CAG的作用机制可能是通过升高胃蛋白酶的含量及促进HSP70的表达,从而发挥对黏膜保护作用。Objective To investigate the effects of Modified Oifan Weitong Granules（QWG） on pepsin content and heat shock protein 70（HSP70） expression in the gastric mucosa of rats with chronic atrophic gastritis（CAG）. Methods CAG rat models were established by means of N-methyl-N＇-nitro-N-nitrosoguanidine（MNNG）, and the effect of QWG on relative indexes was observed and compared with that of Weifuchun Granules. Results Compared with the model group, the atrophy and intestinal metaplasia of gastric mucosa were relieved in large-and medium-dosage QWG groups and in Weifuehun Granules group, and the pepsin content and HSP70 expression were increased in large-, medium-and small-dosage QWG groups and in Weffuchun GranuLes group, the difference being significant（P 〈 0.01 ）. Compared with Weifuchun Granules group, pepsin content and HSP70 expression in large-dosage QWG group were increased with statistical significanee（P〈 0.05）. Conclusion The mechanism of QWG in preventing and treating GAG may be related with prevention of gastric mueosa through increasing pepsin content and HSP70 expression.

关 键 词：慢性萎缩性胃炎 加味七方胃痛颗粒 胃蛋白酶 热休克蛋白70 

分 类 号：R285.5[医药卫生—中药学]