雷公藤红素对血管平滑肌细胞内游离Ca^(2+)和H^+浓度的影响  

Effect of Tripterine on Concentration of Free Calcium and Hydrogen in Vascular Smooth Muscle Cells

作　　者：陈星[1] 丰美福[2] 朱国英 

机构地区：[1]北京医科大学第一医院心内科,北京100034 [2]中国科学院动物研究所

出　　处：《中国中西医结合杂志》1999年第9期538-540,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：卫生部科研基金!资助课题 (94 1 2 4 8)

摘　　要：目的 :观察雷公藤红素对血管平滑肌细胞 (VSMC)内游离Ca2 + 和H+ 浓度的影响。方法 :采用荧光染色法 ,以激光扫描共聚焦显微镜动态研究了不同浓度的胎牛血清对单个血管平滑肌细胞内游离Ca2 + 和H+ 浓度变化的影响效应。结果 :30 %胎牛血清可以引起细胞内一快速的、持续的游离Ca2 + 浓度升高 ,而 30 %胎牛血清加 0 3mg/L雷公藤红素则可以产生抑制细胞内游离Ca2 + 浓度升高的效应 ;2 0 %胎牛血清可以降低细胞内H+ 浓度 ,0 2mg/L雷公藤红素则可以促进细胞内H+ 浓度升高。结论 :雷公藤红素可以拮抗胎牛血清诱发的血管平滑肌细胞内游离Ca2 + 浓度升高的效应 ,其机理可能与该药物抑制了细胞膜的Ca2 +To investigate the effects of tripterine on concentration of free calcium (Ca 2+ ) and hydrogen(H +) in vascular smooth muscle cells (VSMC). Methods: By using fluorescence staining method, changes of the free calcium and hydrogen in single VSMC were studied with laser confocal scanning analyzer after different concentrations of fetal calf serum (FCS), tripterine and FCS plus tripterine were added into the culture pools respectively. Results: The concentration of intracellular Ca 2+ increased rapidly, sequentially and significantly after addition of 30% FCS (P<0 05), 0.3mg/L tripterine plus 30% FCS had inhibition effect on the concentration of the intracellular Ca 2+ , 20% FCS reduced the concentration of intracellular H + significantly (P<0 05), and 0.2mg/L tripterine caused a significant increasing concentration of H + (P<0 05). Conclusion: Tripterine has an antagonistic action on the increasing concentration of intracellular Ca 2+ induced by FCS, the inhibitory effect of tripterine on the Ca 2+ channel of VSMC membrane may be responsible for this new mechanism.

关 键 词：雷公藤红素 血管平滑肌 钙 氢 

分 类 号：R285.5[医药卫生—中药学]