建立血管壁模型的一种新方法及其应用  

Establishment of a Model of Vascular Wall and Its Application

作　　者：尹鸿操[1] 陈铁镇[1] 洪伟[1] 董玉兰[1] 杨向红[1] 

机构地区：[1]中国医科大学实验病理学研究室,沈阳110001

出　　处：《中国动脉硬化杂志》1999年第3期256-259,共4页Chinese Journal of Arteriosclerosis

摘　　要：为探讨建立血管壁模型的新方法，采用胰蛋白酶和胶原酶处理人胎儿羊膜，将人血管内皮细胞及平滑肌细胞分别培养在羊膜的两侧面，用联胺诱发脂质过氧化，观察单核细胞迁移的情况。结果显示，内皮细胞及平滑肌细胞可分别培养在经过处理的羊膜的两面，以构建成类似于机体的血管壁模型。该模型上的内皮细胞及平滑肌细胞发生脂质过氧化后，大量单核细胞迁移入平滑肌细胞层。通过此模型可观察如单核细胞等其它血细胞在血管壁内的情况，该构建血管壁模型的新方法对血管壁生理、动脉粥样硬化及炎症等方面的研究都很有意义。Aim To establish a model of vascular wall and to introduce its application . Methods The human umbilical vein endothelial cells (ECs) and aortic smooth muscle cells (SMCs) were cultured sepparately on the two surfaces of a amnion membrane treated with trypsin and collagenase and the effect of lipid peroxidation (LPx) on the monocyte chemotactic protein (MCP-1) expression in the above mentioned cells, was studied immunocytochemically. Monocyte migration through the ECs layer into the SMCs layer was observed under microscope. Results ECs and SMCs could be cultured on two surface of amnion to build a vascular wall model. After incubation with diamide (DM), the ECs and SMCs on the amnion membrane expressed MCP-1, and resulted in significantly increased monocyte migration through ECs and the amnion membrane into the SMCs layer. Conclusion This study established a model of vascular wall which is a very useful tool for the study on blood vessel biology, atherosclerosis and inflammation.

关 键 词：血管壁 模型 动脉粥样硬化 新方法 应用 

分 类 号：R331.32[医药卫生—人体生理学] R543.5[医药卫生—基础医学]