不同氟烷基因型猪脂肪组织中perilipin基因及糖皮质激素作用相关基因的表达  

Expression of perilipin and glucocorticoids relative regulating genes in adipose tissue of pigs with different halothane genotypes

作　　者：张晓威[1] 梁静[1] 夏东[1] 赵茹茜[1] 杨晓静[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2011年第1期84-88,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30700583);江苏省青年科技创新人才启动项目(BK2007577);教育部博士点新教师基金项目(20070307037)

摘　　要：为研究脂滴包被蛋白(Perilipin)在猪脂肪组织中的表达分布情况及其与糖皮质激素作用的关系,选取氟烷基因隐性纯合子猪(Halnn)和氟烷基因显性纯合子猪(HalNN)为动物模型,采用半定量实时荧光PCR(rqRT-PCR)技术分析其背脂和肾周脂中perilipin基因及脂肪代谢和糖皮质激素作用相关基因的表达。结果显示:两种氟烷基因型猪的体重、背膘厚和背膘指数无显著差异,但Halnn肾周脂重(P=0.09)和肾周脂指数(P=0.10)有低于HalNN猪的趋势。双因子方差分析结果表明Halnn猪脂肪组织中perilipin mRNA水平显著高于HalNN猪(P<0.05),而糖皮质激素受体基因(GR)表达水平显著低于HalNN猪(P<0.05),11β-羟基类固醇脱氢酶1基因(11β-HSD1)、脂肪酸合成酶基因(FAS)和激素敏感酯酶基因(HSL)表达水平在两种基因型之间无显著差异。背脂中perilipin和GR的表达水平与肾周脂中的无显著差异,背脂中11β-HSD1和FAS表达水平显著高于肾周脂(P<0.05),而HSL在背脂中的表达情况正好相反(P<0.05)。以上结果提示:HalNN与Halnn猪脂肪组织中perilipin基因表达存在显著差异,且这种差异可能与GR有关;背脂和肾周脂的脂肪代谢模式可能存在差异。This study was aimed to investigate expression of perilipin in adipose tissue of pigs and its regulation by action of glucocorticoids.The subcutaneous and kidney adipose tissue was taken from pigs with different halothane genotypes（HalNN,homeozygous wild type and Halnn,homozygous mutant）followed by determining the expression of perilipin gene and other related regulating genes with relative quantitative real time PCR（rqRT-PCR.The results showed that there were no significant difference between two halothane genotype pigs in body weight,back fat thickness and its index,but Halnn pigs exhibited lower tendency in kidney fat weight and its index（P=0.09 and P=0.01,respectively）than that in HalNN pigs.Two-way variance analysis results showed perilipin mRNA expression was significantly higher（P0.05）,but lower GR mRNA expression in adipose tissue of Halnn pigs than that in HalNN pigs（P0.05）.There were no significant differences between two halothane genotype pigs in 11β-HSD1,FAS and HSL mRNA expression.Between subcutaneous and kidney fat depots,significant differences were found for 11β-HSD1,FAS and HSL mRNA expression but not for GR and perilipin.These results suggest that perilipin mRNA expression exhibits significant halothane genotype difference,and this difference may be related to GR,meanwhile difference may be exist for fat metabolism between subcutaneous fat and kidney fat.

关 键 词：猪 perilipin基因 背脂 肾周脂 氟烷基因 

分 类 号：S852.21[农业科学—基础兽医学]