8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响  

Effects of eight sulfated polysaccharides on infecting chicken embryo fibroblast of Newcastle disease virus

作　　者：王君敏[1] 胡元亮[1] 张帆[1] 王德云[1] 赵晓娜[1] 张靖[1] 刘静[1] 赵彪[1] 郝丽华 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095 [2]中国兽医药品检查所,北京100081

出　　处：《南京农业大学学报》2011年第1期118-122,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30871887);国家科技支撑计划项目(2008BADB4B06)

摘　　要：用氯磺酸-吡啶法修饰当归多糖(CAPS)和枸杞多糖(LBPS),得到4种硫酸化当归多糖sCAPS0.6、sCAPS1.1、sCAPS1.9、sCAPS2.2和4种硫酸化枸杞多糖sLBPS0.7、sLBPS1.1、sLBPS1.5和sLBPS1.9。分别以CAPS和LBPS为对照,采用MTT法比较了这8种硫酸化多糖对新城疫病毒感染鸡胚成纤维细胞能力的影响。结果表明:硫酸化修饰能显著提高当归多糖和枸杞多糖的抗病毒活性,且与硫酸基取代度有一定的相关性。sCAPS2.2、sLBPS1.5、sCAPS1.9和sLBPS1.9的活性较好,可以作为进一步研究的材料。Chinese angelica polysaccharide（CAPS）and lycium barbarum polysaccharide（LBPS）were modified by chlrulfonic-pyridine method to obtain four sulfated CAPS,sCAPS0.6,sCAPS1.1,sCAPS1.9 and sCAPS2.2,and four sulfated LBPS,sLBPS0.7,sLBPS1.1,sLBPS1.5 and sLBPS1.9.The effects of eight sulfated polysaccharides on cellular infectivity of Newcastle disease virus（NDV）were compared by MTT method taking the non-modified CAPS and LBPS as controls.The results showed that sulfated modification could significantly enhance the anti-virus activity of CAPS and LBPS,which was correlated with the degree of sulfation of sulfated polysaccharide.sCAPS2.2,sLBPS1.5,sCAPS1.9 and sLBPS1.9 possessed better activity and would be as the materials for further research.

关 键 词：硫酸化修饰 当归多糖 枸杞多糖 新城疫病毒 感染细胞能力 鸡胚成纤维细胞 

分 类 号：S853.73[农业科学—临床兽医学]