ET-ISJ标记的开发及陆地棉遗传图谱构建  

Development of Exon-Targeted Intron-Exon Splicing Conjunction(ET-ISJ) Marker and Establishment of Upland Cotton Genetic Map

作　　者：林刚[1] 张轲[1] 张建[1] 滕中华[1] 张正圣[1] 

机构地区：[1]西南大学农学与生物科技学院/农业部生物技术与作物品质改良重点开放实验室,重庆400716

出　　处：《作物学报》2011年第1期87-94,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30871556;30900910);国家高技术研究发展计划(863计划)项目(2006AA10Z1D3)资助

摘　　要：根据植物结构基因外显子拼接位点的保守序列,设计扩增外显子的ET-ISJ(exon targeted intron-exon splice junction)标记引物。利用1 280对ET-ISJ引物组合,对陆地棉品种渝棉1号和T586进行多态性筛选,获得69对多态性引物组合,占引物组合的5.4%。用多态性ET-ISJ引物组合检测(渝棉1号×T586)F2:7重组近交系群体,得到70个位点。以70个ET-ISJ标记位点与523个SSR、59个IT-ISJ、29个SRAP和8个形态标记进行连锁分析,构建的遗传连锁图谱包括59个连锁群和673个位点(68个ET-ISJ、510个SSR、58个IT-ISJ、29个SRAP和8个形态标记)。连锁图覆盖3 216.7 cM,约占棉花基因组的72.3%,标记间平均距离为4.8 cM。68个ET-ISJ标记分布于20条染色体。研究表明ET-ISJ标记多态性较高、稳定性好,可有效用于棉花与其他植物遗传连锁图谱构建。Cotton is the leading fiber crop in the world,and upland cotton contributes over 95% of cotton production.The genetic map of upland cotton is far from saturated,so it is necessary to develop new markers for genetic map construction.ET-ISJ（exon targeted intron-exon splice conjunctions） marker primers were designed according to the conserved intron-exon splicing junction sequences.A total of 1 280 ET-ISJ primer combinations were used to screen polymorphism between upland cotton cultivar Yu-mian 1 and T586,and 69 of which showed polymorphism,accounting for 5.4% of total primer combinations.The 69 polymorphic ET-ISJ primer combinations were used to genotype 270 F2：7 recombinant inbred line derived from（Yumian 1 × T586）,and 70 ET-ISJ loci were obtained.Linkage analysis was conducted on 70 ET-ISJ,523 SSR,59 IT-ISJ,29 SRAP and 8 morphological loci,and a linkage map including 59 linkage groups and 673 loci（68 ET-ISJ,510 SSR,58 IT-ISJ,29 SRAP and 8 morphological loci） was established.The linkage map covered 3 216.7 cM,accounting for 72.3% of cotton genome,with an average interval of 4.8 cM between two markers.Sixty-eight ET-ISJ loci were located on 20 chromosomes.The present study demonstrated that ET-ISJ markers were stable,highly polymorphic,and able to be effectively applied in genetic map construction of cotton and other plant＇s.

关 键 词：陆地棉 外显子定靶内含子-外显子剪接位点标记 遗传图谱 

分 类 号：S562[农业科学—作物学]