正交试验优选川贝母核苷的提取工艺  

Studies on optimum extraction technology for total nucleoside from Fritillaria cirrihosa by orthogonal experiment

作　　者：王丽芝[1] 段宝忠[1] 黄林芳[1] 陈士林[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国医院药学杂志》2011年第3期191-193,共3页Chinese Journal of Hospital Pharmacy

基　　金：科技部支撑计划项目(编号:2006BAI09B05-3);中央级公益性科研院所科研业务专项项目(编号:1381);2007中医药行业专项基金(编号:200707007;200807020)

摘　　要：目的:研究川贝母核苷类成分的最佳提取工艺。方法:以核苷总量的提取率为考察指标,采用L9(34)正交试验对川贝母核苷类的提取工艺进行了研究,考察了提取温度、提取时间、甲醇和水的体积比、溶剂用量对其含量的影响,并用高效液相色谱法进行了验证。结果:10倍量的水在25℃条件下超声提取60min为最佳提取条件。结论:该方法简单合理、稳定性好,是川贝母核苷类成分的最佳提取工艺。OBJECTIVE To optimize the extraction technology for total nucleoside from Fritillaria cirrihosa D. Don. METHODS Some factors, such as the temperature, the time of extraction,the ratio of volume of methanol and water and the volume of solvent, were optimized by orthogonal test. RESULTS The optimum extraction technology was 60 rain at 25℃ with 10 mL water. CONCLUSION The optimum extraction technology was reasonable and stable.

关 键 词：川贝母 核苷 正交试验 

分 类 号：R284.2[医药卫生—中药学]