流动注射化学发光免疫分析检测猪肉中的盐酸克伦特罗残留  

Determination of clenbuterol residual in pork by flow injection chemiluminescence immunoassay

作　　者：陈丽莉[1] 章竹君[1] 付爱华[1] 

机构地区：[1]陕西师范大学,陕西省生命分析化学重点实验室,西安710062

出　　处：《分析试验室》2011年第2期6-8,共3页Chinese Journal of Analysis Laboratory

基　　金：中央高校基础研究基金项目(编号GK20091004)资助

摘　　要：设计了一种快速检测猪肉中盐酸克伦特罗的流动注射-化学发光免疫分析方法。采用过氧化脲作为luminol-CH4N2O.H2O2-HRP化学发光体系氧化剂,联苯硼酸作为增强剂,流动注射化学发光法检测竞争免疫反应后的上清酶标复合物。在最优条件下,测定克伦特罗的线性范围0.05～9μg/L(r=0.9959),检出限为0.02μg/L。该方法已经成功应用于猪肉中克伦特罗残留的检测。A sensitive and rapid immunoassay based on flow injection chemiluminescence detection has been developed for the determination of clenbuterol residual in pork. The chemiluminescence reaction of luminal and hydrogen peroxide-Urea adduct catalyzed by horseradish peroxidase （HRP） can be enhanced by 4-Biphenylboronic acid. After competitive immnnoreaction, the supernate enzyme labeled compound was detected by flow injection chemiluminescence. Under the optimal conditions, the linear range was from 0. 05 to 9μg/L （r = 0. 9959 ）, and the detection limit was 0.02 μg/L. The proposed method has advantages on sensitivity, simplicity, reliablity as well as cheapness on instrument, and has been successfully applied to the determination of clenbuterol residual in pork with satisfactory results, providing a useful monitoring method for food safety.

关 键 词：盐酸克伦特罗 流动注射化学发光 免疫分析 过氧化脲 联苯硼酸 

分 类 号：O657.3[理学—分析化学]