三波长分光光度法测定金银花及叶中总黄酮  

Determination of the contents of total flavonoids in lonicera japonica and its leaves by three wavelength spectrophotometry

作　　者：王柯[1] 王勇[1] 赵东保[1,2] 李明静[1] 刘绣华[1,3] 许平辉 

机构地区：[1]河南大学化学化工学院环境与分析科学研究所,开封475004 [2]济源职业技术学院,济源454650 [3]河南省天然药物与免疫工程重点实验室,开封475004 [4]新乡博凯生物技术有限公司,新乡453000

出　　处：《分析试验室》2011年第2期28-31,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省科技厅国际合作项目(08430051002)资助

摘　　要：以57,-二羟基-6,4'-二甲氧基黄酮为对照品、AlCl3作为显色剂,建立了三波长分光光度法测定金银花及叶中总黄酮含量的方法。总黄酮的质量浓度在1.92～19.20μg/mL范围内与吸光度呈良好的线性关系,相关系数r=0.9997;平均回收率分别为96.6%9,7.1%,RSD=1.4%,2.3%(n=4)。该方法可校正混浊背景倾斜度的影响,还能消除重叠吸收曲线的干扰,适用于植物中总黄酮的测定。A three wavelength spectrophotometry for the determination of the contents of total flavonoids in Lonicera japonica and its leaves was studied by using 5,7-dihydroxy-6,4＇-dimethoxyflavone as a reference substance and aluminum trichloride as a colorimetrie reagent. The linear range of the method was 1.92 - 19. 20 μg/mL with the correlation coefficient of r = 0. 9997. The average recoveries were 96. 6%, 97. 1%, and RSDs were 1.4%, 2. 3% （n = 4）. By selecting the three wavelengths appropriately, the absorbance of interfering components can be corrected efficiently. The method is feasible for the determination of the contents of total flavonoids in plants.

关 键 词：金银花 总黄酮 三波长分光光度法 

分 类 号：O657.3[理学—分析化学]