基于聚亚甲基蓝和磁性/多孔壳纳米CoFe_2O_4/SiO_2固定酶的葡萄糖生物传感器  

Glucose biosensor based on a poly(methlene blue) and CoFe_2O_4/SiO_2 colloid modified platinum electrode

作　　者：娄方明[1] 盛云云[1] 李群芳[1] 

机构地区：[1]遵义医学院药学院,遵义563003

出　　处：《分析试验室》2011年第2期36-39,共4页Chinese Journal of Analysis Laboratory

基　　金：贵州省科技厅科学技术基金(黔科合J字[2010]2214号);遵义医学院硕士科研启动基金(2008F-305)资助

摘　　要：用循环伏安法将电子媒介体亚甲蓝电聚合在铂电极表面上,使其表面形成均匀的聚亚甲蓝膜,通过磁铁对磁性纳米材料的吸附作用将磁性纳米CoFe2O4/SiO2吸附在聚亚甲蓝表面,接着先后吸附葡萄糖氧化酶和磁性纳米CoFe2O4/SiO2,最后用亚甲蓝包埋电极,从而制得葡萄糖氧化酶生物传感器。实验结果表明,该葡萄糖传感器的循环伏安图中氧化峰电流与葡萄糖的浓度在1.2×10-7～6.5×10-5 mol/L范围内呈良好线性关系,相关系数r=0.9962,检出限为6.2×10-8mol/L(S/N=3)。A good glucose biosensor was constructed based on a poly （methlene blue） and CoFe2O4/SiO2 nanoparticles modified Pt electrode. Poly （Methlene blue）, CoFe2O4SiO2 nanoparticles and glucose oxidase （GOD） was absorbed on electrode surface in succession. Finally, GOD and CoFe2O4/SiO2 nanoparticles were firmly immobilized on the electrode surface by the entrapment of poly （methlene blue）. The biosensor has a linear responce over the concentraction of glucose in the range of 1.2 × 10^ -7 -6.5 × 10^ -5 mol/L with a detection limit of 6.2 × 10^-8 mol/L and a correlation coefficient of 0. 9962 （ S/N = 3 ）. The biosensor exhibited high sensitivity, good repeatability and long-time stability.

关 键 词：聚亚甲蓝 葡萄糖氧化酶 磁性纳米CoFe2O4/SiO2 葡萄糖生物传感器 

分 类 号：O657.1[理学—分析化学]