转双抗虫基因741杨对靶标害虫主要解毒酶和乙酰胆碱酯酶的影响  

Effect of transgenic hybrid poplar 741 with two insect-resistant genes on primary detoxification enzyme and acetylcholinesterase of target pests

作　　者：张炬红[1] 高宝嘉[2] 刘军侠[2] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]河北农业大学林学院,河北保定071001

出　　处：《东北农业大学学报》2011年第1期66-70,共5页Journal of Northeast Agricultural University

基　　金：国家自然科学基金资助项目(30070626);河北省自然科学基金资助项目(302312)

摘　　要：用不同系号转基因741杨叶片饲养杨扇舟蛾、舞毒蛾和美国白蛾4～6龄幼虫,测定其中肠重要的两种解毒酶和乙酰胆碱酯酶比活力,研究转基因741杨对靶标害虫生理代谢的影响。结果表明,转基因741杨显著抑制了羧酸酯酶、谷胱苷肽-S-转移酶和乙酰胆碱酯酶的比活力,其中PB11和PB29的抑制作用达到了极显著水平,与转基因741杨抗虫性的生测结果一致。转基因741杨对靶标害虫解毒酶和乙酰胆碱酯酶比活力的抑制作用是其抗虫性的生理机制之一。Fourth-sixth instar larva of Clostera anachoreta （Fabricius）, Gypsy moth and American white moth were fed on various clones of transgenic 741 poplar leaves and the activities of two detoxification enzymes and acetylcholinesterase in midgut were investigated to study the effect of transgenic 741 poplars on the physiological metabolism of the target pests. The results showed that the activities of carboxyleste- rase, glutathione-S-transferase and acetylcholinesterase were inhibited by transgenic 741 poplars, and the inhibition of PB11 and PB29 reached significantly different level. The results were consistent with that of insect-resistant bioassay. The inhibition of transgenic 741 poplars on the activities of detoxification enzymes and acetylcholinesterase of target pests is one of the physiological mechanisms of their insect-resistance.

关 键 词：转基因741杨 靶标害虫 解毒酶 乙酰胆碱酯酶 

分 类 号：S763.42[农业科学—森林保护学]