转DREB1A基因提高烟草抗逆性的研究  

Study on improving stress tolerance ability of tobacco(Nicotiana taba-cum L.) using DREB1A gene

作　　者：李晶[1] 刘煜[1] 蒿连梅[1] 朱延明[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第1期124-129,共6页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅科技项目(10541294)

摘　　要：将拟南芥(Arabidopsis thaliana)DREB1A基因分别在组成型启动子花椰菜病毒35S启动子和逆境诱导型启动子rd29A的驱动下转入烟草中,获得的两种转基因植株对干旱和低温胁迫的抗性均显著提高,但对盐胁迫的抗性无明显变化。研究分析了组成型启动子和逆境诱导型启动子控制的DREB1A转基因植株在形态发育和抗逆性上的差异,对利用DREB1A基因改良作物抗逆性和抗逆基因工程研究中启动子的选择奠定了技术基础。Arabidopsis thaliana DREBIA gene driven by constitutive promoter CaMV 35S and stress inducible promoter rd29A were respectively transformed into tobacco（Nicotiana tabacum L. ）. The obtained transgenic plants using both promoters showed strong drought and cold tolerance, but no salt tolerance was observed. The differences of characteristics between transgenic plants with DREBIA gene under the controlling of constitutive promoter and inducible promoter were analyzed, this will provide the technical basis for utilizing DREBIA gene and the selection of promoter in stress tolerant gene engineering.

关 键 词：DREB1A 烟草 抗逆性 启动子 遗传转化 

分 类 号：S572[农业科学—烟草工业]