肺炎链球菌RNA诱导细胞分泌细胞因子活性的研究  

The secretion of cytokines in human cells induced by Streptococcus pneumococcal RNA

作　　者：肖凤静[1] 马越云[1] 苏明权[1] 杨柳[1] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院检验科,陕西西安710032

出　　处：《中国病原生物学杂志》2011年第1期17-18,21,共3页Journal of Pathogen Biology

摘　　要：目的研究肺炎链球菌RNA对细胞分泌细胞因子的影响。方法提取肺炎链球菌总RNA,分别用不同浓度的RNA作用于肺腺癌细胞A549和巨噬细胞U937,同时以LPS作为对照作用于两细胞,观察细胞分泌细胞因子情况。结果细菌RNA刺激细胞后,其分泌IL-12的能力显著提高且呈剂量依赖关系,当RNA浓度为40μg/ml时,IL-12分泌量提高5倍。而细胞分泌IL-18、TNF-α、IFN-γ的能力与RNA的作用量无直接关系。结论肺炎链球菌RNA具有诱导细胞分泌细胞因子IL-12的能力,是重要的病原相关的分子模式。Objective To study the influence of Streptococcus pneumococcal RNA on the secretion of cytokines in human cell lines.Methods Lung adenocarcinoma A549 cells and U937 macrophages were treated with different concentrations of total RNA extracted from Streptococcus pneumoniae with LPS serving as a control.The level of secretion of cytokines was determined using ELISA.Results IL-12 increased significantly in a dose-dependent manner after stimulation with S.pneumococcal RNA.When the concentration of RNA was 40 μg/mL,the secretion of IL-12 increased 5-fold.Other cytokines such as IL-18,TNF-α,and IFN-γ had no direct association with the role of bacterial RNA.Conclusion As a pathogen-associated molecular pattern,S.pneumococcal RNA has significant capacity to induce the secretion of IL-12 in human cells.

关 键 词：肺炎链球菌 细菌RNA 细胞因子 IL-12 

分 类 号：R378.12[医药卫生—病原生物学]