中国番木瓜枯萎病病原菌的首次鉴定  

First identification on pathogen of papaya wilt disease in China

作　　者：冯岩[1] 陈健 罗金棠 夏恭澍[1] 杜洪忠[3] 

机构地区：[1]仲恺农业工程学院农学院,广东广州510225 [2]广州市果树科学研究所 [3]中国检验检疫科学研究院

出　　处：《植物检疫》2011年第1期55-59,共5页Plant Quarantine

基　　金：广东省重点科技项目(2007A020300002-9);广州市星火计划项目(2007C12E0061)

摘　　要：从发病的番木瓜茎基部组织分离培养得到纯分离物,采用形态学和分子生物学方法进行分离物鉴定。经过致病性测定证实,分离物确定为番木瓜枯萎病的病原菌;通过形态学观察表明,与茄腐镰刀菌(Fusarium solani)形态一致。根据真菌的核糖体DNA(rDNA)内转录间隔区(ITS)的保守性和变异性,采用通用引物ITS1和ITS4进行PCR扩增,然后将PCR产物纯化后直接测序,对测试菌株与10株来自GenBank中的菌株ITS序列进行聚类分析,结果表明,测试菌株与GenBank上注册的茄腐镰刀菌GU134886、GQ451337、EU625405、EF534183和GQ376115聚在同一分枝上,分子鉴定结果与形态学观察结果一致,此为国内首次报道。The pathogens of papaya wilt disease were identified on the basis of pathogenicity testing, as well as morphological characters and molecular identification. Isolates of possible pathogenic fungi were isolated from stems of infected papaya plants. Bases on the pathogenicity experiment, the results showed that the isolate was the pathogen of papaya wilt disease. The morphological identification showed that the isolate was consistent with Fu- sarium solani. By using of conservation and variability of the internal transcribed spacer （ITS） of fungi ribosome DNA, the isolate was amplified by PCR with universal primers of ITS1 and ITS4 and then sequenced. The se- quence from the above isolate was compared with ten sequences published in Genbank database and phylogenitie trees were constructed based on ITS sequences data. The results showed that the tested isolate, GU134886, GQ451337 ,EU625405 ,EF534193 and GQ376115 were clustered into the same clade and all of them were Fusari- um solani. The molecular identification shared the same resuhs with morphology identification. This is the first report that Fusarium solani is the pathogen of papaya wilt disease in China.

关 键 词：番木瓜枯萎病 形态学观察 茄腐镰刀菌 分子鉴定 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治] S41-30[农业科学—植物保护]