球茎甘蓝小孢子培养胚诱导和植株再生研究  

Research on Embryogenesis and Plant Regeneration from Isolated Microspore Culture of Kohlrabi

作　　者：王超楠[1] 闻凤英[1] 刘晓晖[1] 罗智敏[1] 赵冰[1] 黄志银[1] 

机构地区：[1]天津科润蔬菜研究所,天津300384

出　　处：《长江蔬菜》2011年第2期11-13,共3页Journal of Changjiang Vegetables

基　　金：天津市科技支撑计划重点项目(08ZCKFNC01900);天津市自然科学基金项目(10JCYBJC08700);天津市农科院院长基金项目(08008)

摘　　要：以10个球茎甘蓝品种(系)为试材,采用游离小孢子培养方法,研究胚状体发生及其再生植株获得方法。研究结果表明,添加适量6-BA不仅使胚诱导成功率达到了100%,还显著提高了出胚率;胚状体发育程度和整齐度与基因型及每皿出胚数有关;子叶期为最佳的转接时期;MS+0.5 mg/L 6-BA+0.1 mg/L NAA是最适的成苗培养基。Isolated microspore culture was carried out by ten cultivar（strains） of Kohlrabi.It was found that adding suitable concentration of 6-BA could not only raise the embryo induction success to 100%,but also improve inducing rate remarkably.Genotypes and the number of embryos per dish had some effect on development of embryos.Cotyledon period is the optimal stage to transfer to plantlet regeneration medium.＂MS＋0.5 mg/L 6-BA＋0.1 mg/L NAA＂ was the optimal plantlet regeneration medium.

关 键 词：球茎甘蓝 小孢子培养 胚状体 植株再生 

分 类 号：S635.2[农业科学—蔬菜学]