何首乌饮对衰老大鼠脑组织细胞Rb/p53信号转导通路影响  

Effect of Heshouwuyin on Rb/p53 Signal Transduction Pathway in Brain Tissue Cells of Aging Rat

作　　者：韩广明[1] 高晓兰[1] 乔建勇[1] 杨新民[1] 牛嗣云[2] 高福禄[3] 

机构地区：[1]承德医学院第二临床学院,河北承德067000 [2]河北大学基础医学部,河北保定071000 [3]河北师范大学生命科学学院,河北石家庄050016

出　　处：《河北医学》2011年第1期1-4,共4页Hebei Medicine

基　　金：国家自然基金资助项目(30572432)

摘　　要：目的:检测Rb、p16、p53、p19、p21在衰老大鼠脑组织中的表达,探讨何首乌饮抗大鼠脑组织衰老的机制。方法:选用8周龄SD大鼠84只,体重180-220g,随机分为正常组、模型组、何首乌饮高、中、低剂量组、何首乌丸组、自然恢复组,每组12只,采用D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型。免疫组织化学、W estern blotting和RT-PCR法检测pRb、p16、p53、p19、p21在大鼠脑组织的表达差异。结果:模型组大鼠脑组织Rb、p53、p16、p19、p2 l表达明显增加,pRb表达明显减少(P<0.05);何首乌饮、何首乌丸组能显著提高pRb的表达,降低Rb、p53、p16、p19、p2 l表达(P<0.05),何首乌饮高剂量组调节作用大于其他各组(P<0.05)。结论:何首乌饮调节脑组织Rb/p53信号转导通路相关基因和蛋白表达起到延缓衰老的作用。Objective： To investigate the mechanism of Heshouwuyin in anti-aging in brain of the aging model rats by detecting expression of Rb,p16,p53,p19 and p21 in aging rats brain.Method： Totally 84 of 8 month SD rats,weight 180-220g,were selected and randomly divided into normal group,model group,Heshouwuyin high,middle,low group,Heshouwuwan group and spontaneous recovery group with 12 rats in each group.The sub·acute aging rats were made by injecting D·gal into abdominal cavity continually.SP immunohistochemistry,Western blotting and RT-PCR were used to observe the differential expression of phosphorylated Rb protein,p16,p53,p19 and p21 in brain tissue.Result： The expression of brain tissue Rb,p53,p16,p19 and p2l in model group rat obviously increased,the expression of pRb obviously decreased（P0.05）;Heshouwuyin and Heshouwuwan group raised the expression of pRb,decreased the expression of Rb,p53,p16,p19,p21（P0.05）,and the regulating effect of Heshouwuyin high group was more effective than rest groups（P0.05）.Conclusion： Heshouwuyin regulates the expression of Rb/p53 signal transduction pathway related gene and protein in brains tissue to defer aging.

关 键 词：何首乌饮 RB p53 脑组织 衰老 免疫印迹法 RT-PCR 大鼠 

分 类 号：R285.5[医药卫生—中药学]