大葱的CPD染色与核型分析  

CPD Staining of Allium fistulosum L. var. giganteurn Makino and Its Karyotype Analysis

作　　者：刘良科[1] 赵丽娟[1] 熊飞[1] 

机构地区：[1]民族药用植物资源研究与利用湖南省重点实验室湘西药用植物与民族植物学湖南省高校重点实验室怀化学院生命科学系,湖南怀化418008

出　　处：《安徽农业科学》2011年第1期35-36,共2页Journal of Anhui Agricultural Sciences

基　　金：怀化学院民族药用植物资源研究与利用湖南省重点实验室资助项目

摘　　要：[目的]研究大葱的CPD带型,为大葱的染色体识别提供标记,了解大葱染色体DNA序列的分子特征。[方法]采用去壁火焰干燥法制备大葱的根尖细胞有丝分裂染色体,用CPD(PI和DAPI组合)对大葱染色体进行染色,结合常规的染色体测量,对大葱进行核型分析。[结果]CPD染色后,大葱的所有染色体的端点区都显示了红色的CPD带;大葱的核型公式为2n=2x=16=14m+2st(SAT)。[结论]根据CPD带,可对大葱的染色体进行更加准确的识别;大葱的所有染色体的端点区都含有GC丰富的DNA序列;CPD染色可为葱属植物的物种鉴定、系统分类与进化等方面的研究提供DNA分子方面的证据。[Objective] The marker for the identification of Allium fistulosum L.var. giganteurn chromosome was provided through the research on the CPD bends so that the molecular character of DNA sequence of its chromosomes could be understood. [Method] The mitotic chromosomes of the root-tip cells of Allium fistulosum L.var.giganteurn Makino was prepared with the wall degradation hypotonic method and the chromosomes were stained by CPD（combination of PI and DAPI） . Then,the karyotype analysis of the materials was conducted combined with conventional measure of the chromosome. [Results] The red CPD bends were showed on all chromosomes endpoint area of tested materials after CPD staining and the karyotype formula of A. fistulosum L.var.giganteurn was 2n=2x=16=14m＋2st（SAT） . [Conclusion] The A. fistulosum L.var.giganteurn chromosome could exactly be identified depending on the CPD bends. The DNA sequence in the endpoint area of all of chromosome was richened with GC. The evidence of DNA molecule in the research on the identification,systematic classification and evolution of Allium sp. could be provided.

关 键 词：大葱 染色体 CPD染色 核型分析 

分 类 号：S633.1[农业科学—蔬菜学]