荧光茧色判性家蚕黄酮合酶Ⅰ(BmFNS Ⅰ)基因在家蚕组织内的表达差异  

Expression Differences of Flavonoid Synthase Ⅰ(BmFNS Ⅰ) in the Tissues of Bombyx Mori with Sex-identified Fluorescent Cocoon Color

作　　者：许乃霞[1] 张雨青[1,2] 沈卫德[1,2] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]苏州大学蚕桑研究所,江苏苏州215123

出　　处：《安徽农业科学》2011年第1期41-43,共3页Journal of Anhui Agricultural Sciences

基　　金：国家重点基础研究发展计划"863"项目(2006AA10A118);江苏省高校自然科学基金项目(07KJB230102)

摘　　要：采用荧光定量PCR分别检测不同品种荧光判性家蚕雌、雄家蚕的中肠、丝腺和脂肪体中的黄酮合酶I(BmFNS I)基因的表达。结果表明,家蚕BmFNS I基因在各品种荧光判性家蚕中肠中的相对表达量最高,其次是脂肪体,在丝腺中的表达量较低,说明BmFNSI基因在对家蚕食物桑叶内的黄酮类化合物的合成代谢中起重要作用;但是在各品种荧光判性家蚕的雌、雄中肠中的表达并无明显差异,说明荧光判性家蚕雌、雄蚕在对桑叶内的黄酮类化合物的合成代谢途径上不存在差异,至少在FNS I基因所调节的黄酮类化合物的合成代谢上不存在差异。Expression differences of Flavonoid synthase Ⅰ（BmFNS Ⅰ） gene in the midgut,silk gland and fat body of the male and female Bombyx mori with sex-identified fluorescent cocoon color was detected by fluorescence quantitative PCR. And the result showed that the relative expression level BmFNS Ⅰ gene in the midgut of the silkwormn was the highest,followed by that in fat body,and the expression level in silk gland was the lowest,indicating that BmFNS Ⅰ gene played an important role in flavonoids anabolism in leaves of mulberry,which was the food of silkworm. However,there was no significant difference in the BmFNS Ⅰ gene expression level in the midgut between male and female Bombyx mori varieties with sex-identified fluorescent cocoon color,suggesting that there was no difference in anabolism of flavonoids in leaves between the male and female Bombyx mori with sex-identified fluorescent cocoon color,at least no difference in anabolism of flavonoids regulated by flavonoid synthase Ⅰ gene.

关 键 词：荧光茧色判性 家蚕 黄酮合酶Ⅰ 组织 

分 类 号：S881.24[农业科学—特种经济动物饲养]