猪繁殖与呼吸综合征病毒GXA毒株人工感染仔猪的病理学研究  

Research on Pathology of Piglets Infected with GXA Strain of PRRSV

作　　者：韦英益[1] 廖素环[1] 李爱友[1] 曾芸[1] 李跃华[1] 袁紫云[1] 宁均淼[1] 黄伟坚[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530003

出　　处：《安徽农业科学》2011年第1期299-301,共3页Journal of Anhui Agricultural Sciences

基　　金：广西大学科研基金(X051013)项目资助

摘　　要：[目的]探讨PRRSV-GXA毒株对断奶仔猪的致病性。[方法]将12头PRRSV/PCV2抗体和抗原阴性的健康仔猪随机分为感染组(9头)和对照组(3头),感染组通过口服和肌肉注射方式接种PRRSV-GXA株细胞毒(4ml/头),对照组接种无病毒的细胞培养液(4ml/头)。感染后第11、14和21天分别剖杀感染组3头和对照组1头,根据临床症状、病理学变化、特异性抗体水平以及病毒核酸等判断其致病性。[结果]仔猪感染PRRSV后第6～10天体温稍微升高,随后逐渐恢复至正常。早期出现短暂的精神沉郁,轻度厌食但没有出现典型临床症状。剖检的病理变化仅见于感染后第21天,有2头感染仔猪肺膈叶前缘出现小的局灶性间质性肺炎,镜检肺泡壁间质增宽,有多量单核细胞和淋巴细胞浸润。在感染后第9天检测到PRRSV特异性抗体,并随着时间呈上升趋势,感染后第21天达到较高值。在4头感染仔猪的肺脏及肺门淋巴结检测到病毒核酸,其中1头仔猪扁桃体也检测到病毒核酸。[结论]PRRSV-GXA毒株对仔猪的毒力较弱,但能刺激机体产生较高水平特异性抗体。[Objective] To investigate the pathgenicity of piglets infected with PRRSV GXA strain.[Method] A total of 12 4-week-old healthy piglets which were negative of antibody and antigen of PRRSV/PCV2 were randomly divided into challenge group（9 piglets） and control group（3 piglets）.GXA strain of PRRSV（4 ml/head） was inoculated intranasally and intranscularly simultaneously and cell culture medium of non RRRSV（4 ml/head） was inoculated in conteol group.Three piglets（in challenge group ） and one piglet（in control group） were killed individually in 11,14 and 21 d after starting experiment.Pathogenicity was evaluated by temperature detection,clinical symptom,pathological,specific antibody and viral nucleic acid.[Results] Rectal temperature increased lightly at 6 d post-infection and then to regain normal.GXA strain of PRRSV caused no obviously clinical signs.Pathological change was only observed in two infected piglets at 21 d post-infection,which characterized as small focal interstitial pneumonia in lung.The histological lesions of PRRSV infected piglets were characterized by infiltration of macrophages and lymphocytes in alveolar septum.Challenge group produced a higher level of special-antibody titres and PEESV DNA had been detected.[Conclusion]The pathological lesions were milder in piglets infected PRRSV GXA strain.

关 键 词：猪繁殖与呼吸综合征病毒 感染 仔猪 病理学 特异性抗体 

分 类 号：S856.3[农业科学—临床兽医学]