鸭源致病性大肠杆菌的分离鉴定及致病性观察  

The Identification and Pathogenicity of Escherishia coli Isolated from Ducklings

作　　者：蔡双双[1] 张红[2] 徐朋朋[3] 宋恩赐[2] 谷长勤[2] 张万坡[2] 程国富[2] 胡薛英[2] 

机构地区：[1]湖北三峡职业技术学院,湖北宜昌443000 [2]华中农业大学动物医学院,武汉430070 [3]河南科技大学动物科技学院,河南洛阳471003

出　　处：《湖北农业科学》2011年第1期118-121,共4页Hubei Agricultural Sciences

摘　　要：自湖北省荆州市某鸭场送检病料中分离到一株疑似鸭大肠杆菌,经选择性培养基培养、生化试验、PCR鉴定、动物回归试验、药物敏感性试验,确认为鸭大肠杆菌,该菌对多种抗生素具有耐药性,用已分离鉴定的大肠杆菌经腹腔注射途径感染15日龄雏鸭,致病性试验结果显示,经腹腔注射途径感染雏鸭死亡率达100%,引起纤维素性心包炎、肝被膜炎和气囊炎,说明此株大肠杆菌具有强致病性。A suspected E.coli from ducklings was isolated in Jingzhou of Hubei province.After selective culturing,biochemical test,PCR identification,regression test and medicine-resistance test,it was identified to be Escherishia coli from ducklings with high resistance to several antibiotics.Subsequently,some 15 day-old ducklings were infected with the E.coli isolates by lumbar injection for pathogenicity test.The results showed that the mortality of infected ducklings was 100%.In the dead ducks fibrinous pericarditis,glissonities and air sacculitis were also observed.It proved that this E.coli isolate was in high pathogenicity.

关 键 词：鸭 大肠杆菌 分离鉴定 致病性 

分 类 号：S858.32[农业科学—临床兽医学] S852.612[农业科学—兽医学]