组织块培养法和酶消化法分离髌韧带细胞的比较  

Comparison of tenocytes isolated by tissue block culture and enzyme digestion

作　　者：沈晓涛[1,2,3,4] 陈斯韵[1,2,3] 赵宝寅[1,2,3] 郑馨[1,2,3] 蔡冬青[1,2,3,4] 

机构地区：[1]暨南大学,再生医学教育部重点实验室,广东省广州市510632 [2]暨南大学,再生医学香港中文大学暨南大学联合实验室,广东省广州市510632 [3]暨南大学,科技部及广东省科技厅国际科技合作基地,广东省广州市510632 [4]暨南大学,生物医学工程系,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2010年第46期8563-8567,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：863项目(2007AA02Z105);国家自然科学基金(30770886,30570369,30340038,30973158);广东省自然科学基金重点项目(04105826);广东省科技攻关项目(2004B30601007)~~

摘　　要：背景:调动和激活韧带与肌腱细胞群是有效修复损伤韧带与肌腱的关键,通过对韧带与肌腱细胞可能存在的不同群进行分离,对韧带与肌腱组织正常结构的维护及损伤修复具有重要意义。目的:比较经组织块培养法和酶消化法分离出来的髌韧带细胞的形态、增殖,细胞复制性衰老与表面标记物表达。方法:分别用组织块培养法和酶消化法对成年SD大鼠的髌韧带细胞进行分离培养,比较该两种方法得到的髌韧带细胞的形态,生长曲线,表面标记物的表达,细胞复制性衰老等情况。结果与结论:经两种分离方法分离所得的髌韧带细胞的形态、增殖能力、表面标记物及复制性衰老均存在差异。提示髌韧带细胞可能存在不同群,且不同群的髌韧带细胞可能在髌韧带正常功能与修复中发挥不同的作用。BACKGROUND：It is importance to activate tenocytes groups to repair the injured ligament and tendon.So,separating the different groups of tenocytes will elucidate the maintenance and repair of normal ligament and tendon structures.OBJECTIVE：To compare the cell morphology,proliferation,replicative senescence and the cell surface markers expression of tenocytes isolated by tissue block culture and enzyme digestion.METHODS：Tenocytes obtained from patellar tendon were isolated by tissue block culture and enzyme digestion.The cell morphology,proliferation,replicative senescence and the cell surface markers of isolated tenocytes were investigated.RESULTS AND CONCLUSION：There were significant differences between tenocytes isolated by tissue block culture and enzyme digestion in cell morphology,proliferation,replicative senescence and the cell surface markers.It suggested that tenocytes of patellar tendon might include different groups and the individual group of tenocytes may play the unique function in normal tendon function and tendon healing.

关 键 词：韧带 肌腱 损伤 修复 韧带细胞 

分 类 号：R318[医药卫生—生物医学工程]