大鼠磨牙加力移动龈沟液中核因子κB受体活化子配体/骨保护素的表达变化  

Expression variation of the receptor activator for nuclear factor kappa B ligand and osteoprotegerin in the gingival crevicular fluid of rats with orthodontic appliances

作　　者：张昀[1] 杨磊[2] 马钢[1] 张林棋[1] 高巍然[2] 张红霞[1] 

机构地区：[1]白求恩军医学院附属解放军第260医院口腔科,河北省石家庄市050041 [2]解放军264医院,山西省太原市030001

出　　处：《中国组织工程研究与临床康复》2010年第46期8581-8584,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:核因子κB受体活化子配体和骨保护素系统参与破牙骨质细胞的形成并调节牙根吸收过程,而不同力值大小及加力时间下龈沟液中核因子κB受体活化子配体和骨保护素的表达变化是否可以作为判断牙根吸收的量化指标鲜有报道。目的:探讨核因子κB受体活化子配体和骨保护素表达变化与力值大小及加力时间的相关性。方法:雄性SD大鼠随机分成0.49N,0.98N和1.47N力值组,每组按照加力时间分为加力0,3,7,10,14,21及28d亚组。各组大鼠建立大鼠正畸牙模型,采用吸潮纸尖法提取实验牙的龈沟液,应用ELISA法测定不同力值和加力时间龈沟液核因子κB受体活化子配体和骨保护素的浓度及比值变化。结果与结论:加力不同时间点不同力值加载组组间大鼠龈沟液中核因子κB受体活化子配体/骨保护素的浓度比值比较差异均具有显著性意义(P<0.01),力值越大,该比值越大。0.49N加力组在加力的前21d,随着加力时间的延长,大鼠龈沟液中核因子κB受体活化子配体/骨保护素的值逐渐增高(P<0.01);第28天的核因子κB受体活化子配体/骨保护素的比值趋于稳定。0.98N和1.97N加力组,随着加力时间的延长,大鼠龈沟液中核因子κB受体活化子配体/骨保护素的值均持续逐渐增高(P<0.01)。结果表明,大鼠龈沟液中核因子κB受体活化子配体和骨保护素的比值与力值大小及作用时间具有相关性,可用于正畸牙根吸收的临床检测指标。BACKGROUND：Receptor activator of nuclear factor κB ligand ligand（RANKL） and osteoprotegerin（OPG） play an important role in the formation of odontclasts and the root resorption.Whether the expression and changes of RANKL and OPG in gingivalcrevicular fluid（GCF） may be an index of root resorption in orthodontic tooth movement remains unclear.OBJECTIVE：To investigate the changes and significance of the receptor activator for RANKL and OPG in GCF of the rats with orthodontic appliances.METHODS：An animal model of orthodontic tooth movement was established in male SD rat.The rats were randomly divided into0.49 N,0.98 N,and 1.47 N groups,which assigned into 0,3,7,10,14,21 and 28 days subgroups.Following careful sampling ofGCF with absorbed paper points in the rats,the levels of RANKL and OPG were determined by ELISA.The effect of loading duration on RANKL and OPG ratio was observed.RESULTS AND CONCLUSION：There were obviously differences in RANKL/OPG in different groups（P 0.01）.The RANKL/OPG was increased as the force increased.In the 0.49 N-force application group,the level of RANKL/OPG increased significantly during the first 21 days（P 0.01）,but the changes were not significant at 28 days.In the 0.98 N and 1.47 N-force application groups,the level of RANKL/OPG increased significantly between different orthodontic force application days（P 0.01）.RANKL and OPG in GCF play an important role in the root resorption in the rats with orthodontic appliances,which can beserved as an clinical index for testing root resorption.

关 键 词：龈沟液 正畸牙 核因子κB受体活化子配体 骨保护素 牙根吸收 

分 类 号：R318[医药卫生—生物医学工程]