Synoviolin基因修饰滑膜细胞预防肌腱粘连  

Prevention of tendon adhesion using synoviolin gene modified synoviocytes

作　　者：谢志新[1] 刘桔慧[1] 农春花[1] 许卓芳[1] 温秀梅[1] 黄尹亭[1] 

机构地区：[1]南方医科大学附属深圳宝安区人民医院骨科,广东省深圳市518101

出　　处：《中国组织工程研究与临床康复》2010年第46期8639-8642,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：深圳市科技计划项目(200903181);课题名称:Synoviolin基因修饰滑膜细胞预防肌腱粘连的作用研究~~

摘　　要：背景:Synoviolin基因过表达,能使滑膜细胞过度生长和增殖,因此将Synoviolin基因转入膜细胞将有可能实现无滑膜肌腱滑膜化,从而有效防止肌腱粘连的发生。目的:观察Synoviolin基因修饰滑膜细胞预防肌腱粘连的影响。方法:将30只Leghom鸡随机分为实验组与对照组,建立肌腱损伤模型。实验组将含Synoviolin基因重组的腺病毒载体直接注入腱鞘残端表面。术后1,3,8周,行大体观察,组织学检查,生物力学测试。结果与结论:实验组第四趾肌腱缝合处粘连程度、第三趾肌腱的滑动距离及模拟主动屈曲度均优于对照组(P<0.05);与对照组相比,实验组Ⅰ型胶原蛋白含量下降,Ⅲ型胶原蛋白比例下降;说明Synoviolin基因修饰滑膜细胞能使无滑膜肌腱滑膜化,提示利用Synoviolin基因修饰滑膜细胞是屈指肌腱术后预防肌腱粘连的有效方法之一。BACKGROUND：The over expression of synoviolin gene leads to synoviocyte overgrowth and proliferation.Thus,it is possible to realize synovialization of non-synovial tendon by importing synoviolin gene into synoviocytes,which can prevent tendon adhesion.OBJECTIVE：To explore the effects of synoviolin gene modified synoviocyte on the prevention of tendon adhesion.METHODS：Totally 30 Leghom chickens were prepared for tendon injury models and randomly divided into the experimental and control groups.The adenovirus vector containing synoviolin gene was injected into the remnant of tendon sheathes.Gross observation,histological examination and biomechanical test were performed at 1,3 and 8 weeks after operation.RESULTS AND CONCLUSION：The adhesion at the fourth toe,gliding distance of the third toe and stimulate active flexion of the experimental group were superior to those in the control group（P 0.05）.Compared with the control group,the type Ⅰ collagen content was decreased in the experimental group,and the ratio of type Ⅲ collagen was declined.The findings demonstrated that synoviolin gene modified synoviocyte could transform the nonsynovial membrane tendon into a synovial membrane tendon,suggesting that it is an effective method for preventing flexor tendon from adhesion after operation.

关 键 词：滑膜细胞 肌腱粘连 Synoviolin基因 软组织构建 肌肉肌腱组织工程 

分 类 号：R318[医药卫生—生物医学工程]