小鼠胎肝中胎肝激酶-1阳性细胞具有血管祖细胞的功能特性  

FLK-1 cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells

作　　者：金成豪[1] 米满吉和[2] 丁伟荣[1] 居石克夫[2] 

机构地区：[1]江西省人民医院血液内科,南昌330006 [2]日本九州大学医学研究院病理病态学研究室

出　　处：《中华心血管病杂志》2010年第12期1108-1112,共5页Chinese Journal of Cardiology

基　　金：基金项目：国家自然科学基金（30860115）;日本学术振兴会基础研究C

摘　　要：目的 探讨胎肝中胎肝激酶-1（fetla liver kinase 1,FLK-1）阳性细胞的特征和功能.方法 免疫磁珠分离胎肝FLK-1阳性细胞,流式细胞术（又称荧光活化细胞分类计数,fluorescence activated cell sorter,FACS）检测胎肝FLK-1阳性细胞.体外生长因子诱导培养、测定FLK-1阳性细胞的分化、增殖活性.FLK-1阳性细胞植入75%肝切除的小鼠肝脏模型中,借助荧光解剖显微镜及免疫组织化学方法观察血管形成及细胞分化的情况.结果 FLK-1阳性细胞经生长因子诱导后,可表达典型的血管内皮细胞、平滑肌细胞的标志.75%肝切除的小鼠肝脏模型中,植入的FLK-1阳性细胞在肝脏的纤维囊中分化成血管细胞并形成血管.结论 胎鼠肝脏FLK-1阳性细胞具有血管祖细胞的功能,具有潜在的临床应用价值.Objective To characterize the differential and proliferative activities of FLK-1＋ cells derived from mouse fetal liver. Method The FLK-1＋ fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1 ＋ cells into the inferior pole of the spleen of nine-week-old male C57BL/6 mice after two-thirds hepatectomy. Results In response to growth factors, FLK-1 ＋ cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1 ＋ cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver. Conclusion These results indicate that FLK-1 ＋ cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cellbased therapy.

关 键 词：血管内皮生长因子受体2 干细胞 细胞分化 细胞移植 

分 类 号：R686[医药卫生—骨科学]