特应性皮炎患者外周血单个核细胞、血清及泪液中MIF检测的临床意义  

The expression of macrophage migration inhibitory factor in PBMC,serum and lacrimal fluid of patients with atopic dermatitis and its clinical significance

作　　者：马蕾[1] 陈世义[2] 李艳阳[3] 舒春梅[1] 张俊花[1] 

机构地区：[1]滨州医学院附属医院皮肤科,256603 [2]吉林大学第一医院皮肤科 [3]沈阳市第七人民医院皮肤科

出　　处：《中华检验医学杂志》2010年第12期1155-1159,共5页Chinese Journal of Laboratory Medicine

基　　金：山东省自然科学基金资助项目（ZR2010H0.013）;山东省高等学校科技计划项目资助课题（J10LF90）;滨州医学院科研启动基金（BY2009KYQD03）

摘　　要：目的 探讨MIF在AD患者PBMC、血清及泪液中的表达水平及其在AD诊断中的临床意义.方法 AD患者按SCORAD指数分3个亚组,轻度AD组11例、中度AD组23例、重度AD组9例;并用实时定量RT-PCR检测43例AD患者和31名健康对照者（健康对照组）PBMC中MIFmRNA的表达水平,用ELISA法检测血清和泪液中MIF含量.结果 AD组PBMC中MIF mRNA的表达水平为7.46（3.38～8.90）,明显高于健康对照组[1.67（1.24～2.45）,Z=-6.141,P〈0.05];轻、中、重度AD组MIF mRNA的表达水平分别为2.35（2.12～2.49）、7.83（6.54～8.90）和8.76（8.22～9.73）,中、重度AD组均显著高于健康对照组（Z值分别为-6.237、-4.520,P均〈0.05）.AD组血清中MIF的含量为36.32（11.89～43.80）μg/L,明显高于健康对照组[7.89（6.13～9.54）μg/L,Z=-6.180,P〈0.05];轻、中、重度AD组血清中MIF的含量分别为8.98（7.90～10.51）μg/L、36.50（29.78～43.23）μg/L、45.70（41.27～48.84）μg/L,中、重度AD组均显著高于健康对照组（Z值分别为-6.238、-4.521,P均〈0.05）.AD组泪液中MIF的含量为12.66（2.01～20.12）μg/L,较健康对照组[0.85（0.77～1.06）μg/L]明显升高（Z=-4.118,P〈0.05）;轻、中、重度AD组泪液中MIF的含量分别为1.10（0.83～1.35）μg/L、12.66（9.76～15.87）μg/L、24.65（19.29～30.94）μg/L,中、重度AD组均显著高于健康对照组（Z值分别为-4.062、-3.372,P均〈0.05）.中、重度AD组PBMC中MIF mRNA表达水平及血清、泪液中MIF的含量与疾病严重程度SCORAD指数均呈明显正相关（r值分别为0.395、0.404、0.515,P均〈0.05）.结论 不同AD病程患者PBMC、血清和泪液中MIF表达增高,MIF可作为判断AD病情活动和评价AD疾病严重程度的有效检验指标.Objective To investigate the expression of MIF in PBMC, sera and lacrimal fluid samples in patients with AD, and study the diagnostic significance of MIF in AD. Methods Forty-three AD patients （11 mild AD patients,23 moderate AD patients, 9 severe AD patients classfied by SCORAD index）and 31 age- and sex-matched healthy control subjects were recruited. Real-time RT-PCR was employed to analyze the expression of MIF mRNA in PBMC. ELISA was performed to detect the concentrations of MIF in sera and lacrimal fluid samples. Results AD patients had significantly higher levels of MIF mRNA in PBMC than normal controls [7.46 （3.38-8.90） vs 1.67 （ 1.24-2.45 ）, Z=-6.141, P ＜ 0.05]. The concentrations of MIF in sera and lacrimal fluid samples in AD patients were also markedly higher than those of normal controls [serum 36. 32（ 11.89-43.80） μg/L vs 7.89（6.13-9.54） μg/L, Z = -6.180,P 〈0.05; lacrimal fluid 12.66（2.01-20.12） μg/L vs 0.85（0.77-1.06） μg/L, Z = -4.118,P 〈0.05]. MIF mRNA levels were 2.35 （ 2.12-2.49 ） , 7.83 （ 6. 54-8.90 ） and 8.76 （ 8.22-9.73 ） in mild, moderate and severe AD respectively, and the expression was higher in moderate and severe AD than in normal controls （Z= -6.237, -4.520,P ＜0.05）. MIF serum concentrations were 8.98（7.90-10.51） μg/L, 36.50 （29.78-43.23） μg/L and 45.70（41.27-48. 84） μg/L in mild, moderate and severe AD respectively, and the differences were significant in moderate and severe AD compared to normal controls （ Z = - 6.238,- 4.521, P 〈 0.05 ）. The lacrimal fluid MIF oncentrations were 1.10 （ 0.83-1.35 ） μg/L, 12.66 （ 9.76-15.87） μg/L and 24. 65 （ 19.29-30.94） μg/L in mild, moderate and severe AD respectively. Similarly,they increased significantly in moderate and severe AD compared to normal controls （Z = -4.062,- 3.372, P ＜ 0.05 ）. In moderate and severe AD, MIF mRNA levels in PBMCs and MIF concentrations in sera and lacrimal fluid samples were all positively correlated with the severity of A

关 键 词：特应性皮炎 巨噬细胞游走抑制因子 逆转录聚合酶链反应 酶联免疫吸附测定 

分 类 号：R686[医药卫生—骨科学]