表达HIV-1 B亚型env基因的质粒DNA及腺病毒载体疫苗的免疫原性研究  

Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene

作　　者：杨海儒[1] 张凌斐[2] 冯霞[1] 余双庆[1] 庄著伦[1] 李红霞[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052 [2]北京工业大学生命科学与生物医学工程学院病毒与药理室

出　　处：《中华实验和临床病毒学杂志》2010年第6期415-417,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重大科技专项（2008ZX10001-009）;国家自然科学基金（30872397）

摘　　要：目的 构建表达中国B亚型HIV-1流行株env基因的DNA及重组腺病毒载体疫苗,将其用于预防或治疗HIV感染.方法 构建质粒DNA疫苗pVR-gp160及重组腺病毒载体疫苗rAdV-gp160.将这两种疫苗以不同的方式免疫BALB/c小鼠,分别采用ELISPOT方法 和ELISA方法 检测免疫小鼠中HIV-1 Gp120特异性细胞免疫反应及抗体反应.结果 DNA疫苗单独免疫及DNA疫苗初免/腺病毒疫苗加强免疫的联合免疫方案皆可诱导较高水平的Gp120特异性细胞免疫反应;而在体液免疫方面,各实验组产生的Gp120特异性抗体水平都较低.结论 所构建的DNA疫苗及rAdV疫苗能有效表达Gp160蛋白,并可有效激活机体的细胞免疫反应.Objective To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines. Methods The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively. Results DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups. Conclusion DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.

关 键 词：艾滋病疫苗 基因 env 质粒 腺病毒科 

分 类 号：R3[医药卫生—基础医学]