氡与香烟烟雾染毒诱发BEAS-2B细胞的体外转化  

In vitro transformation of immortalized human bronchial epithelial cells BEAS-2B induced by radon and cigarette smoke

作　　者：杜厚兵[1] 童建[1] 聂继华[1] 刘海云[1] 张素萍[1] 吴昭昭[1] 李建祥[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室,215123

出　　处：《中华放射医学与防护杂志》2010年第6期634-638,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（30671784）

摘　　要：目的 通过氡与香烟烟雾体外染毒诱发细胞恶性转化,建立氡与吸烟致肺癌发生的细胞模型.方法 将永生化人支气管上皮细胞系BEAS-2B分为对照组（C组）、氡染毒组（Rn组）、烟雾染毒组（Sm组）、联合染毒组（Rn＋Sm组）,细胞以每孔1 × 105个接种于Transwell培养皿,氡或香烟烟雾持续泵入直接对细胞染毒.检测细胞恶性转化情况.结果 BEAS-2B细胞经氡及香烟烟雾和联合染毒后发生形态学改变,细胞生长失去接触抑制,染氡组、烟雾染毒组、联合染毒组第5染毒代细胞传代20代后获得对血清诱导末端分化的抗性,克隆形成率相对值由0.31±0.18上升至1.92±0.27、2.03±0.14、2.95±0.60,同时该代细胞锚着独立性生长能力也由（0.01±0.02）%上升至（4.89±0.30）%、（8.36±0.50）%、（11.74±0.69）%.染氡组、烟雾染毒组、联合染毒组第5染毒代细胞传代20代后凋亡率由（11.76±0.17）%降低至（4.62±0.42）%、（8.63±0.15）%、（3.68±0.33）%.结论 氡与香烟烟雾单独及联合染毒均能诱发BEAS-2B细胞体外转化,且联合染毒导致损伤效应更加显著,可作为研究氡及吸烟致肺癌的细胞模型.Objective To establish a model of malignant transformation of human cells in vitro to study the lung cancer induced by radon and cigarette smoke. Methods The immortalized human bronchial epithelial cells BEAS-2B were divided into control group（ C ）, radon group （ Rn）, cigarette smoke group （Sm） and combined group （Rn-Sm）. Cells were planted onto transwell membrane one day before exposure and were directly exposed to radon and cigarette smoke pumped in a gas inhalation box. After the exposure cells were trypsinized into dishes for further growth and malignancy transformation phenotype was detected in order to compare the effects due to radon and cigarette smoke exposure. Results BEAS-2B cells showed malignantly transformed phenotype by exposure to radon and cigarette smoke. A series of sequential steps emerged among transformed cells, including altered growth kinetics, resistance to serum has changed from 0. 31 ± 0. 18 to 1.92 ± 0. 27,2. 03 ± 0. 14,2.95 ± 0. 60, and anchorage-independence growth increased from （0.01 ±0.02）% to （4.89 ±0.30）%,（8.36 ±0.50）%,（11.74 ±0.69）%.After being subculture for 20 generations, cell apoptosis of the fifth generation cells exposed to radon,cigarette smoke and both was significant decreased from （ 11.76 ± 0. 17 ） % to （4. 62 ± 0. 42 ） %、 （ 8.63 ±0. 15 ）%、 （3.68 ± 0. 33 ）%. Conclusions BEAS-2B cells could be malignancy transformed by radon and cigarette smokein vitro, which could be used as a cell model in lung bronchial carcinogenesis.

关 键 词：氡 香烟烟雾 体外染毒 BEAS-2B细胞 恶性转化 

分 类 号：R114[医药卫生—卫生毒理学]