^3H-TdR与^125Ⅰ-UdR掺入淋巴细胞增殖的比较  

Comparison of ^3H-TdR and ^125Ⅰ-UdR incorporation on the proliferation effect of lymphocytes

作　　者：万建美[1] 张友九[1] 范我[1] 朱然[1] 宁萍[1] 崔凤梅[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院江苏省放射医学与防护重点实验室

出　　处：《中华放射医学与防护杂志》2010年第6期658-660,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：江苏省卫生厅医学科技发展基金（H200341）;苏州市社会发展科技计划资助（SS0416）

摘　　要：目的 比较3H-TdR与125Ⅰ-UdR掺入淋巴细胞的增殖效应.方法 用3H-TdR与125Ⅰ-UdR掺入法测定淋巴细胞和Daudi淋巴瘤细胞的增殖效应.结果 3H-TdR和125Ⅰ-UdR在正常淋巴细胞中的掺入率分别为20.95%±1.06%和1.00%±0.04%,在Daudi淋巴瘤细胞中的掺入率分别为29.94%±4.10%和6.02%±0.73%.3H-TdR在细胞中的掺入率明显高于125Ⅰ-UdR;且3H-TdR和125Ⅰ-UdR在淋巴瘤细胞中的掺入率高于正常淋巴细胞.结论 就淋巴细胞而言,作为示踪剂125Ⅰ-UdR不能替代3H-TdR;但对于淋巴瘤细胞,能否代替3H-TdR有待于进一步研究.Objective To compare the incorporation method of 3H-TdR and 125Ⅰ-UdR on determining the proliferation effect of lymphocytes. Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125Ⅰ-UdR incorporation. Results The incorporating fraction of 3H-TdR and 125Ⅰ-UdR into lymphocyte was 20.95% ± 1.06% and 1.00% ±0.04%,respectively, and the incorporating fraction for the lymphoma cells was 29. 94% ± 4. 10% and 6. 02% ±0. 73% ,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125Ⅰ-UdR, but the incorporating fractions of 3H-TdR or 125Ⅰ-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125Ⅰ-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125Ⅰ-UdR could be replace 3H-TdR or not needs further research.

关 键 词：淋巴细胞 淋巴瘤细胞 3H-TDR 125Ⅰ-UdR 掺入率 

分 类 号：R817[医药卫生—影像医学与核医学]