TGF-β1对病理性瘢痕成纤维细胞中β-catenin表达的影响及意义  

Effect of TGF-β1 on the expression of β-catenin in fibroblasts of the pathological scar and its significance

作　　者：肖明[1] 郭澍[1] 王晨超[1] 孙强[1] 

机构地区：[1]中国医科大学附属第一医院整形外科,辽宁沈阳110001

出　　处：《中国美容整形外科杂志》2010年第12期759-762,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 探讨TGF-β/Smad信号转导通路与Wnt/β-catenin信号转导通路在病理性瘢痕发病机制中的作用及相互关系.方法 随机选取瘢痕疙瘩（K组）、增生性瘢痕（H组）和正常皮肤组织（N组）各3例,进行原代成纤维细胞培养,用不同浓度的TGF-β1处理细胞,应用免疫组化技术检测β-catenin在各组中的表达变化.结果 β-catenin在各组中的表达水平随TGF-β1浓度变化,呈先递增后递减趋势,三者β-catenin表达变化幅度为K组＜H组＜N组,K组成纤维细胞与H组成纤维细胞相比,差异无统计学意义（P＞0.05）,而N组成纤维细胞与K组、H组成纤维细胞相比,差异有统计学意义（P＜0.01）.结论 TGF-β/Smad信号转导通路与Wnt/β-catenin信号转导通路联合作用,导致病理性瘢痕形成.Objective To explore the interaction and interrelationship between the TGF-β/Smad signal pathway and Wnt/β-catenin signal pathway in pathogenesis of pathological scar. Methods Three cases of keloid （ K group）, 3 of hyperplastic scar （ H group） and 3 of normal skin （ N group ）were selected randomly, and then the fibroblasts taken from the 3 groups were performed the primary culture at different TGF-β1 concentrations and the expression of β-catenin was detected by immunohistochemisty technique. Results The expression of β-catenin in each group increased firstly and then decreased with the concentration changes of TGF-β1. The expression changes of the β-catenin in the three group were K group 〈 H group 〈 N group ,and there was no statistical significance in the variance between K group and H group（ P 〉 0.05 ）. There was substantial statistical significance in the variance between N group and K group, H group（ P 〈0.01 ）. Conclusion The unification of GF-β/Smad signal pathway and Wnt/β-eatenin signal pathway is an important factor in the formation of pathological scar.

关 键 词：Β-连环蛋白 病理性瘢痕 成纤维细胞 动态表达 

分 类 号：R619.6[医药卫生—外科学]