意大利工蜂不同发育时期抗氧化酶基因mRNA表达量的变化  

Changes in mRNA expression level of antioxidant enzyme genes in worker bees of Apis mellifera ligustica(Hymenoptera:Apidae) at different developmental stages

作　　者：肖培新[1] 吴在富[1] 刘昭华[1] 胥保华[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《昆虫学报》2010年第11期1202-1206,共5页Acta Entomologica Sinica

基　　金：现代农业蜂产业技术体系建设专项资金(NYCYTX-43);公益性行业(农业)科研专项(200903006)

摘　　要：超氧化物歧化酶(SOD)和过氧化氢酶(CAT)在清除工蜂体内自由基的过程中起着关键的作用,其活性和基因表达量的高低与蜜蜂的寿命、抗病力有直接的联系,进而影响着蜂群的生产性能。本研究采用实时荧光定量PCR法研究意大利蜜蜂Apis mellifera ligustica不同发育时期铜锌超氧化物歧化酶(copper zinc superoxide dismutase,CuZnSOD)、锰超氧化物歧化酶(manganese superoxide dismutase,MnSOD)及过氧化氢酶(catalase,CAT)基因mRNA表达量的变化。实验分别在幼虫、蛹、成虫3个阶段5个时间点(产卵后5d的幼虫,产卵后14d的蛹,出房后1d,12d,32d的成虫)取样。结果表明,3个抗氧化酶基因在各个发育阶段均有表达,其中CuZnSOD和MnSOD mRNA的表达量在幼虫到蛹期和出房后12-32d两个阶段呈下降趋势,而都以出房后1d时表达量最大,蛹期表达量最小,且显著低于其他4个时期(P<0.05)。CuZnSOD基因mRNA的表达量在成虫阶段的下降趋势不明显,MnSODmRNA的表达量则显著下降(P<0.05);而CAT基因的mRNA表达量以蛹期最低,且其表达量随蜜蜂的生长发育稳定上升,在出房后12d和32d时表达量显著高于其他3个时期。这些研究结果在分子水平上揭示了抗氧化酶基因在蜜蜂不同发育时期的作用。The superoxide dismutase and catalase play an important role in protecting worker bees from reactive oxygen species,and their enzyme activities and expression levels are associated with honeybee longevity and disease resistance which affect the performance of the colony.This study was to observe the developmental regulation of CuZnSOD,MnSOD and CAT genes in Apis mellifera ligustica worker bees at mRNA level by real-time quantitative PCR,so as to provide a theoretical basis for the study of aging and disease resistance of honeybees.During the larval,pupal and adult stages,we collected the samples of larva（5 d after egg laying by the queen）,pupa（14 d after egg laying by the queen） and adult（1 d,12 d,32 d after emergence）,respectively.The results showed that these three antioxidant enzyme genes were expressed in all developmental stages.Both CuZnSOD and MnSOD mRNA expression levels decreased during larval to pupal stages and the adult stage at 12-32 d after emergence,with the highest level at 1 d adult after emergence and the lowest level in pupa.However,no significant decrease was found in the expression of CuZnSOD gene at the adult stage,while the expression of MnSOD gene decreased obviously.The lower mRNA level of the CAT gene was detected in pupa.However,the CAT gene expression steadily increased during the development of honeybees,and the expression at 12-32 d adult after emergence was significantly higher than those at the other stages（P0.05）.The study illustrates the function of antioxidant enzymes at the molecular level during different developmental stages of A.mellifera ligustica worker bees.

关 键 词：意大利蜜蜂 抗氧化酶 MRNA表达量 发育时期 实时荧光定量PCR 

分 类 号：Q966[生物学—昆虫学]