家兔门静脉自发节律性收缩机制的初步探讨  

Preliminary Study on the Mechanism of Spontaneous Rhythmic Contraction in Rabbit Portal Vein

作　　者：黄旭[1] 赵丹[2] 王佐妤[1] 张明亮[2] 韩燕飞[1] 陆红丽[1] 严志强[2] 姜宗来[2] 许文燮[1] 

机构地区：[1]上海交通大学医学院生理学教研室,上海200240 [2]上海交通大学力学生物学与医学工程实验室,上海200240

出　　处：《生物医学工程学杂志》2010年第6期1211-1215,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10732070;10672103)

摘　　要：为了探讨门静脉自发节律性收缩的起搏机制,本文应用免疫组织化学与全细胞膜片钳技术研究了家兔门静脉间质细胞(ICs)的形态及电生理学特性。经过酶解分离得到的家兔门静脉单个IC呈星状或纺锤状,自胞体向外伸出很多毛刺状突起,其酪氨酸激酶受体c-Kit染色呈阳性。应用全细胞记录模式,膜电位钳制在-60 mV,在该类细胞上记录到了自发性节律性内向电流,其频率与门静脉自发性收缩频率相近。这种电流对L型钙通道阻断剂尼卡地平不敏感,可被非选择性阳离子通道阻断剂氯化钆阻断。结果提示,在新鲜分离的家兔门静脉ICs上记录到频率与门静脉自发性收缩相近的自发性内向电流,这种内向电流可能就是启动门静脉自发性收缩的起搏电流。This study sought to probe into the mechanism of spontaneous contraction of portal vein.The morphological and electrophysiological characteristics of the freshly isolated interstitial cells（ICs） of rabbit portal vein were investigated by using immunohistochemical and conventional whole-cell patch clamp techniques.The isolated interstitial cells exhibited stellate-shaped or spindle-shaped bodies with a variable number of thin processes projecting from cell bodies,and these cells were noted to be c-Kit immunopositive.Under conventional whole-cell patch clamp configuration,the membrane potential was held at-60mV,the spontaneous rhythmic inward currents were recorded in ICs,and the frequencies of which were similar to those of spontaneous contraction of portal vein.The inward currents were insensitive to nicardipine（an L-type calcium channel blocker） but could be abolished by gadolinium（a non-selective cation channel blocker）.The results suggested that the spontaneous rhythmic inward currents recorded in freshly isolated ICs may be pacemaker currents which elicit the spontaneous contraction of portal vein.

关 键 词：门静脉 间质细胞 自发性内向电流 起搏电流 

分 类 号：R331.32[医药卫生—人体生理学]