小鼠胎盘组织细胞移植的抗衰老作用研究  

Research on Anti-aging Effect of Mouse Placenta Cells Transplantation

作　　者：李军[1] 张洹[1] 刘革修[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《生物医学工程学杂志》2010年第6期1312-1316,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30670902);广东省自然科学基金资助项目(06300546);国务院侨办重点学科建设基金资助项目(200510)

摘　　要：为研究小鼠胎盘细胞同种异体移植的抗衰老作用,将15月龄雌性BALB/c老年小鼠分为对照组(A)、短期移植组(B)以及长期多次移植组(C)共3组;取孕18 d小鼠胎盘,50目细胞筛网研磨胎盘,制备小鼠胎盘细胞悬液(1×107cells/ml),经腹腔注入B组和C组小鼠体内,1 ml/只,每10 d注射1次,连续注射3次。之后,再继续对C组小鼠不定期多次注射胎盘细胞悬液,直至实验结束。移植3个月后评价B组小鼠心脏结构和功能的各指标,心脏质量指数(心脏质量/体质量)和脾脏质量指数(脾脏质量/体质量),心脏、肾脏、肺脏、皮肤等器官组织学衰老程度分值,血清总超氧化物歧化酶活力、丙二醛含量以及谷胱甘肽过氧化物酶活力,皆优于A组(均P<0.05);且B组小鼠存活日期为A组的1.7倍,而B、C组之间无统计学差异;同时,Y染色体原位杂交实验表明,B组和C组小鼠多个脏器内均可检测到长期存活的移植细胞。结果提示:移植小鼠胎盘细胞可在一定程度上延缓小鼠衰老进程。To determine the role of placenta cells allogeneic graft in mammalian longevity,the 15-month-old female BALB/c mice（n=50） were divided into Control group（A）,Short-term transplanted group（B） and Long-term transplanted many times group（C）.Their placentae（at 18 days of gestation） were taken out and ground with 50-eye cell grit,and the cells were intraperitoneally injected into the mice of B group and C group three times at intervals of 10 days;then the cells were transplanted into the mice of C group many times till the time of death.The mice were evaluated by use of ultrasound-cardiogram;autopsy;score of cardia,spleen,skin,lung,kidney;histopathology;serum total superoxide dismutase activity,serum maleic dialdehyde content,and serum glutathione peroxidase activity.The long-term surviving stem cells were found to be located in many organ tissues of B and C groups＇ mice with in situ Y chromosomal hybridization dyeing.Median life span of B group mice was 1.7 times that of A group＇s after transplantation,but there was no statistically significant difference between B group and C group.Three months after transplantation,in B group,the pathological developments of significant skin,cardia,lung,and kidney were delayed;the retrogradation of heart function was attenuated;the data on heart mass index（mass of heart/mass of body）,left ventricular mass and serum Maleic Dialdehyde content,and on spleen mass index（mass of spleen/mass of body）,left ventricular diastolic volume,serum Total Superoxide Dismutase activity and serum Glutathione Peroxidase activity,were all in a direction favourable to B group （P0.05）.These results were in line with the hypothesis,i.e.longevity can be enhanced to some extent by transplantation of placenta cells.

关 键 词：胎盘 移植 抗衰老 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]