电针刺激对脑缺血大鼠内源性EPCs及相关血清细胞因子的影响  

The Effect of Electro-acupuncture on Endogenous EPCs and Serum Cytokines in Cerebral Ischemia-Reperfusion Rat

作　　者：赵瑛[1] 陈斯佳[2] 于文娟[2] 蔡绍皙[2] 张莉[3] 王秀志[3] 唐安科[1] 

机构地区：[1]重庆师范大学重庆市动物生物学重点实验室,重庆400047 [2]重庆大学生物工程学院,重庆400044 [3]北京中医药大学针灸学院,北京100029

出　　处：《生物医学工程学杂志》2010年第6期1322-1326,共5页Journal of Biomedical Engineering

基　　金：重庆师范大学;重庆市动物学重点学科拓展研究资助项目

摘　　要：用线栓法阻塞大鼠大脑中动脉(MCA)制备局灶性脑缺血/再灌注(MACO)模型,观察内源性内皮祖细胞(EPCs)及其相关细胞因子在电针改善脑缺血中的作用。实验证明:MACO模型稳定性高,电针"曲池"、"足三里"穴后,大鼠神经功能恢复较对照组快;脑缺血再灌注损伤提高了外周血中内皮祖细胞(EPCs)的数量,以24 h最为明显,这可能与损伤引起的炎症反应及机体自身修复有关;针刺可在一定程度上降低诱导型一氧化氮合成酶(iNOS)的活性,减轻脑缺血的炎症反应、减缓损伤,同时调节外周血中EPCs的数量,再灌注后24 h明显增加,48 h最为明显(P<0.01);脑缺血后大鼠血清中血管内皮细胞生长因子(VEGF)含量有所升高,反映了脑缺血刺激可以一定程度上激发机体的应激反应,针刺使血中VEGF含量更高,推测这种VEGF的高表达可能对EPCs起到了动员作用,促使梗死后的EPCs的趋化、归巢。此外,在调节外周血EPCs过程中,基质金属蛋白酶(MMP-9)、碱性成纤维细胞生长因子(bFGF)等细胞因子也发挥了相应作用。总之,针刺通过动员外周血中EPCs数量、降低iNOS活性和VEGF的高表达,从而促进血管新生来改善脑缺血,这可能是针刺治疗脑缺血诸多途径之一。In this research project,rats were made into animal models of acute focal cerebral ischemia and reperfusion（IR） by occlusion of their middle cerebral artery（MCAO）.We observed the effect of endogenous endothelial progenitor cells（EPCs） and serum cytokines on cerebral ischemia rats treated by electro-acupuncture（EA）.The results showed： MCAO model had high stability after EA treatment which was delivered via the acupuncture needles inserted into ＂quchi＂ and ＂zusanli＂ points,the nervous functions of cerebral IR rats recovered faster than those of rats not treated;EPCs in rats＇ blood increased after acute focal cerebral ischemia and reperfusion;and the growth rate was obvious in IR group.This phenomenon might be related to the inflammation elicited by injury of ischemia and self-repair. Besides,EA treatment could decrease induced nitric oxide synthase（iNOS） activity,alleviate injury after cerebral ischemia,and regulate the quantity of EPCs in blood.The quantity of EPCs in blood increased in IR-24hr.In IR-48hr,the rise of EPCs quantity was significant（P0.01）.The level of vascular endothelium growth factor（VEGF） in serum of rats after cerebral ischemia was escalated,which indicated to a certain extent that cerebral ischemia could stimulate stress reaction.EA treatment could raise VEGF level,which suggested that high expression of VEGF could accelerate mobilization,chemotaxis and homing of EPCs.At the same time,the levels of matrix metalloproteinase-9（MMP-9） and basic fibroblast growth factor（bFGF） also changed.In conclusion,EA treatment could promote neovascularization after cerebral ischemia by mobilizing EPCs,decreasing iNOS activity and increasing VEGF level.This may be one of the ways by which EA could treat cerebral ischemia.

关 键 词：电刺 脑缺血 内皮祖细胞 血清细胞因子 

分 类 号：R245[医药卫生—针灸推拿学]