基于无细胞蛋白表达的蛋白质微阵列技术  

The Cell-free Protein Synthesis-based Protein Microarray Technology

作　　者：吕林莉[1] 刘必成[1] 

机构地区：[1]东南大学肾脏病研究所/附属中大医院,南京210009

出　　处：《生物医学工程学杂志》2010年第6期1397-1400,1409,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30570476);江苏省自然科学基金重点资助项目(BK2007709)

摘　　要：高密度蛋白质微阵列构建的主要瓶颈之一是蛋白质的来源和蛋白质在载体表面的稳定性问题。传统的蛋白质微阵列构建依赖于对大量蛋白的分别表达、纯化和固定,这种方法费时费力,且不易维持蛋白质的稳定性。无细胞蛋白表达技术在蛋白质微阵列构建中的应用,使得目标蛋白可以在载体表面直接从固定的DNA和RNA模板合成,克服了传统构建方法的局限。本文简要介绍无细胞蛋白表达的蛋白质微阵列技术及其应用研究进展。The major bottle-neck in the way of constructing high density protein microarray is the availability and stability of proteins.The traditional methods of generating protein arrays require the in-vivo expression,purification and immobilization of hundreds or thousands of proteins.The cell-free protein array technology employs cell-free expression systems to produce proteins directly onto surface from co-distributed or pre-arrayed DNA or RNA,thus avoiding the laborious and often costly processes of protein preparation in the traditional approach.Here we provide an overview of recently developed novel technology in cell free based protein microarray and their applications in protein interaction analysis,in antibody specificity and vaccine screening,and in biomarker assay.

关 键 词：无细胞蛋白合成 蛋白质微阵列 蛋白质组学 蛋白质芯片 生物标志物 

分 类 号：Q503[生物学—生物化学]