云锡矿粉诱导人肺上皮细胞转化过程中FHIT蛋白和Ki-67抗原的表达及意义  

Expression of fragile histidine triad （FHIT） protein and Ki-67 in transformed epithelial cells induced by Yunnan tin mine dust

作　　者：边莉[1] 何永文[2] 付红梅[1] 阮永华[3] 高倩[3] 王春艳[1] 金克炜[3] 

机构地区：[1]昆明医学院第一附属医院病理科,650031 [2]昆明医学院第一附属医院附属口腔医院,650031 [3]昆明医学院第一附属医院病理教研室,650031

出　　处：《中华劳动卫生职业病杂志》2010年第12期895-899,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30560053,81060189）;云南省应用基础研究项目资助（2008CD009,2006C0042M）

摘　　要：目的 探讨云锡矿粉诱导人肺上皮细胞转化过程中脆性组胺酸三联体（FHIT）蛋白和Ki-67抗原的表达及意义.方法 选用100 μg/ml的云锡矿粉染毒永生化人支气管上皮细胞BEAS-2B（B）及人胚肺成纤维细胞WI-38（W）,每次染毒72 h,隔代染毒直至第9代.自第11代起将上述细胞分组共培养.B100表示100μg/ml矿粉诱导的B细胞,W100表示100 μg/ml矿粉诱导的W细胞.分组如下：B组;B（W）组：与W共培养的B细胞;B（W100）组：与W100共培养的B细胞;B100组：100 μg/ml矿粉诱导的B细胞;B100（W）组：与W共培养的B100细胞;B100（W100）组：与W100共培养的B100细胞.应用免疫细胞化学法检测第16、26及36代各组上皮细胞中FHIT蛋白和Ki-67抗原的表达,以Ki-67蛋白阳性细胞数计算细胞增殖指数（PI）.结果 BEAS-2B上皮细胞中存在FHIT蛋白表达,各代B组、B（W）及B（W100）组细胞FHIT蛋白表达水平无明显差异.第16代,B100组细胞FHIT蛋白表达水平低于B组细胞,B100（W）组及B100（W100）组FHIT蛋白表达水平较B100组进一步降低.第26代,B100、B100（W）及B100（W100）组细胞FHIT蛋白表达水平进一步降低,而至第36代,经矿粉诱导转化的上皮细胞重现FHIT蛋白的表达,且B100组、B100（W）组、B100（W100）组表达强度呈递增趋势.第16、26及36代未经矿粉诱导的各组肺上皮细胞Ki-67阳性细胞数PI均＜3%.第16代,B100组、B100（W）组及B100（W100）组细胞Ki-67阳性细胞PI分别为4.40%、4.97%和7.87%,第26代时分别增加为7.33%、7.87%和12.66%,第36代时分别进一步提高为12.60%、19.73%和24.80%.与B100、B100（W）组比较,第16、26、36代B100（W100）组阳性细胞PI明显增高,差异有统计学意义（P＜0.05）.结论 FHIT可能是云锡矿粉诱导BEAS-2B上皮细胞转化的一个作用靶点,云锡矿粉诱导可导致BEAS-2B上皮细胞Ki-67阳性细胞数增加,增殖活性增强.Objective To study the expression and significance of fragile histidine triad（FHIT）and Ki-67 in transformed epithelial cells induced by Yunnan tin mine dust.Methods Every second generation of immortalized human bronchial epithelial cells（BEAS-2B）and human embryo lung fibroblasts（WI-38）were exposed to 100 μg/ml Yunnan tin mine dust for 72 h, until the ninth generation.The cells were subsequently co-cultured from the 11th generation.Experimental setup： B group,B（W）group,B（W 100）group, B100 group,B100（W）group, B100（W100）group.The expressions of FHIT and Ki-67 in epithelial cells were determined by the method of immunocytochemistry at the 16th, 26th and 36th generation.The percentage of Ki-67 positive cells was calculated as proliferation index.Results The expression of FHIT was observed in BEAS-2B cells.The expression levels of FHIT among B group, B（W）group and B（W 100）group had not instinctive difference.At the 16th generation, the expression of FHIT in the B100 group was decreased compared with that in the B group and the expression of FHIT between B100（W）group and B100（W100）group was lower than that in the B100 group.At the 26th generation, the expression of FHIT was decreased compared with that at the 16th generation in the B100,B100（W）and B100（W100）groups.However, At the 36th generation, positive expression were observed again in the B 100, B 100（W）and B 100（W 100）groups and the expression levels were in incremental order.At the 16th, 26th and 36th generation, the proliferation indexes of B group, B（W）group and B（W 100）group were all ＜3%.The proliferation indexes of B100, B100（W）and B100（W100）were increased step by step with the generation elongation.Conclusions FHIT could be a target at which Yunnan tin mine dust induces transformation of BEAS-2B cells.The proliferation activation of BEAS-2B cells can be improved by Yunnan tin mine dust.

关 键 词：粉尘 基因表达 抗原 

分 类 号：R686[医药卫生—骨科学]