黄瓜‘新津春四号’遗传转化体系的建立  

Establishment of Genetic Transformation System of Cucumber 'New Jinchun 4'

作　　者：许娜[1] 储俊[1] 夏海武[1] 赵岩[1] 钱立生[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《热带亚热带植物学报》2010年第6期679-684,共6页Journal of Tropical and Subtropical Botany

基　　金：安徽省教育厅自然科研一般项目(KJ2010B294;KJ2010B052)资助

摘　　要：对根癌农杆菌介导‘新津春四号’黄瓜(Cucumbis sativus L.)遗传转化的影响因素进行了研究。结果表明,黄瓜叶片适宜的潮霉素(Hygromycin,Hyg)筛选浓度为40 mg L-1;500 mg L-1羧苄青霉素(Carbenicillin,Carb)可有效抑菌。预培养2 d有利于转化;共培养2 d有利于提高转化频率并避免农杆菌的过度生长;添加150μmol/L乙酰丁香酮(Acetosyringone,AS),农杆菌浸染液pH5.7、浓度0.8,浸染时间8 min为最佳遗传转化条件。再生植株经gus基因的瞬时表达检测及PCR检测证明hpt基因已成功转化。The genetic transformation system of cucumber ＇New Jinchun 4＇ mediated by Agrobacterium tumefaciens were studied.The cucumber leaves as explants were pre-cultured on MS＋1.0 mg L-1 6-BA＋0.15 mg L-1 IAA for 2 days.After infected by Agrobacterium（OD600=0.8,pH=5.7） for 8 min supplemented with 150 μmol/L acetosyringone（AS）,the explants were co-cultured on MS ＋ 1.0 mg L-1 6-BA ＋ 0.15 mg L-1 IAA ＋ 500 mg L-1 Carb ＋ 40 mg L-1 Hyg for 2 days.The hpt gene were transformed successfully to regenerated plantlets by gus instantaneous expression and PCR test.

关 键 词：根癌农杆菌 黄瓜 遗传转化 

分 类 号：S642.2[农业科学—蔬菜学]