多花黄精组织培养快繁技术的研究  

Study on Rapid Micropropagation Technology by Tissue Culture of P.cyrtonemaHua.

作　　者：刘红美[1] 方小波[1] 夏开德[1] 杨苏文[1] 

机构地区：[1]贵阳医学院医学生物技术教研室,贵州贵阳550004

出　　处：《种子》2010年第12期13-17,共5页Seed

基　　金：贵州省高层次人才科研条件特助经费(Q2005-4;187);贵阳医学院青年基金(k2007-48)

摘　　要：[目的]:建立多花黄精组织培养快速繁殖体系。[方法]:以多花黄精带芽根茎为外植体,消毒后将其置于富含不同激素配比的培养基中培养,筛选各阶段合适的培养基。[结果]:在附加有2,4-D的诱导培养基上出现不定芽,增殖培养以MS+6-BA4.0 mg/L+2,4-D0.2 mg/L为好,增殖倍数可达10倍。在增殖培养基中加入GA3有利于壮苗。6-BA、2,4-D、GA3组合更加有利于形成粗壮无根苗。培养基1/2 MS+IBA0.7 mg/L最适合用于诱导黄精不定芽生根,生根率可达95%。[结论]:2,4-D比NAA更有利于多花黄精的不定芽诱导。该繁殖体系可在短时间内提供大量黄精种苗。[Objective]：To develop a rapid micropropagation system by tissue culture for P.cyrtonemaHua.[Methods]：The rhizomes that had sprouted of P.cyrtonemaHua as explants were cultured on MS medium supplemented with different concentrations of BA in combination with different concentrations of NAA or 2,4-D to select optimized medium for different phase.[Results]：Multiple shoots were only got in medium supplemented with 2,4-D.The solid MS medium with 6-BA（2.0mg/L） and 2,4D（0.1mg/L） was suitable for the multiplacation of shoots and the propagation times could reach to 10,the same medium supplemented with GA3（0.5mg/L） was suitable to strongen shoots.The MS medium with IBA（0.7mg/L） was suitable for the shoots rooting and 95% plantlets with roots were obtained.[Conclusions]：2,4D was better than NAA to induce adventitious bud.The rapid micropropagation system could be used to develop more P.cyrtonemaHua seedings in short time.

关 键 词：多花黄精 组织培养 快繁体系 

分 类 号：S567.23[农业科学—中草药栽培]