一株新型寄生溶藻细菌的分离及初步鉴定  

Isolation and preliminary identification of a novel parasitic algicidal bacteria

作　　者：许丽娜[1] 程凯[1] 许敏[1] 赵以军[1] 杨季芳[2] 陈吉刚[2] 吴庆喜[2] 

机构地区：[1]华中师范大学城市水环境生态学湖北省重点实验室,武汉430079 [2]浙江万里学院宁波市微生物与环境工程重点实验室,浙江宁波315100

出　　处：《华中师范大学学报（自然科学版）》2010年第4期658-661,665,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家海洋局公益性项目(200705014);国家科技重大专项(2009ZX27105-001);湖北省自然科学基金项目(2008CDA101)

摘　　要：从宁波象山港分离得到1株具有溶藻能力的细菌,命名为XD-G.该菌株对中肋骨条藻(Skeletonema costatum 1115)的溶藻效果较好,感染1 d后,藻细胞基本裂解,叶绿素a减少达66.5%.在透射电子显微镜下观察菌株XD-G感染9 h后的中肋骨条藻切片,发现藻细胞内有大量细菌出现,由此推测,菌株XD-G是1种寄生性溶藻细菌.菌株XD-G不但能裂解中肋骨条藻及热带骨条藻(Skeletonema troicum),而且对赤潮异弯藻(Heterosigma akashi wo)也有一定的溶藻效果.An algae-lysing bacteria called XD-G was isolated from XiangShan bay in NingBo. The algicidal effect of the bacterium XD-G on S. costatum 1115 is significant. almost all of the algal cells were lysed in 24 hours, and the concentration of Chl a was reduced by 66.5% then. By electron microscope observation, a large number of the bac- terium could be observed in S. costatum cells after 9 hours＇ infection, which means that the bacteria XD-G is a kind of parasitic bacteria. Furthermore, the bacteria XD-G could not only lyse S. costatum 1115 and Skeletonema troicum, but also remove Heterosigma akashiwo.

关 键 词：赤潮 溶藻细菌 中肋骨条藻 寄生 

分 类 号：X55[环境科学与工程—环境工程]