维生素C对心肌缺血/再灌注家兔心功能和SOD、MDA的影响  

Effects of Vitamin C on Superoxide Dismutase and Malondialdehyde in Myocardium and Cardiac Function of Rabbits during Ischemia/Reperfusion

作　　者：杨鸿雁[1,2] 彭艳玲[1] 刘兴德[1] 

机构地区：[1]贵阳医学院附院心血管科,贵州贵阳550004 [2]贵阳医学院

出　　处：《贵阳医学院学报》2010年第6期555-557,共3页Journal of Guiyang Medical College

基　　金：贵州省高层次人才科研条件特助经费项目TZJF(2006)12号

摘　　要：目的:观察维生素C(VitC)对心肌缺血/再灌注(I/R)家兔心功能和心肌组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的影响。方法:家兔24只随机分为3组,假手术组(A组)、心肌缺血/再灌注组(B组)、VitC组(C组);用生物机能实验系统记录各组家兔左室收缩峰压(LVSP)、左室舒张末压(LVEDP)和左室内压最大收缩/舒张变化速率(±dp/dtmax);用黄嘌呤氧化酶法测定SOD,硫代巴比妥酸法测定MDA含量。结果:与A组比较,B、C两组的±dp/dtmax减低,有显著性差异(P<0.01);与B组比较,C组±dp/dtmax增高,有统计学差异(P<0.05);与A组比较,B、C两组心肌组织SOD活性减低,而MDA含量增高(P<0.05或P<0.01);与B组比较,C组心肌组织中SOD活性增高,而MDA含量降低(P<0.05)。结论:VitC能够改善心肌I/R家兔的心功能,其机制可能与抑制机体内脂质过氧化有关。Objective： To investigate the effects of vitamin C（VC） on contents of superoxide dismutase（SOD）,and malondialdehyde（MDA） in myocardium and cardiac function of rabbits during ischemia/reperfusion（I/R）;Methods： Twenty four rabbits were divided randomly into 3 groups（8 in each）： sham-operated group（group A）,I/R group（group B）,and VC treated group（group C）.Organism function experiment system was used for recording left ventricular systolic pressure（LVSP）,left ventricular end diastolic pressure（LVEDP）,and maximal changing rate of left ventricular inner pressure（±dp/dtmax）;SOD in myocardium of rabbits was detected with xanthine oxcydation method and MDA detected with thiobarbituric acid method.Results： ± dp /dtmax in groups B and C were lower than that in group A（P〈0.01）,but ± dp /dtmax in group C was higher than that in group B（P〈0.05 or P〈0.05）.SOD in myocardium was lower and MDA was higher in group B and group C than those in group A（P〈0.01）,while SOD content was higher and MDA was lower obviously in group C than those in group B（P〈0.05）.Conclusions： VC could improve cardiac function in rabbits during I/R.The mechanism might relate to reduction of over-oxidative reaction of lipid.

关 键 词：维生素类 心肌再灌注损伤 心室功能 超氧化物歧化酶 丙二醛 兔 

分 类 号：R541[医药卫生—心血管疾病]