槲寄生碱对人肝癌细胞株SMMC-7721 bcl-2mRNA表达的影响  

Effect of Mistletoe Alkali on Expression of bcl-2 mRNA in Human Hepatocarcinoma Cell SMMC-7721

作　　者：孔凡青[1] 丁海麦[1] 杨艳红[2] 郭美祥[2] 

机构地区：[1]包头医学院,内蒙古包头014010 [2]包头医学院第一附属医院,内蒙古包头014010

出　　处：《天津农业科学》2010年第6期8-9,共2页Tianjin Agricultural Sciences

摘　　要：为研究槲寄生碱对人肝癌细胞株SMMC-7721对bcl-2mRNA表达的影响。采用半定量RT-PCR法测定bcl-2mRNA表达变化。结果表明,RT-PCR检测SMMC-7721细胞中bc1-2、mRNA表达的相对强度,发现bc1-2mRNA的表达强度降低(P<0.05)。说明中药槲寄生碱抑制肝癌SMMC-7721细胞增殖作用与降低bcl-2mRNA表达水平有关。This study was designed to investigate the expression of bcl-2 mRNA in Human Hepatocarcinoma cell line SMMC-7721 tread with mistletoe alkali in vitro. Change of bcl-2 mRNA expression in human Hepatocarcinoma cell SMMC-7721 was detected by Reverse transcription polymerase chain reaction （RT-PCR）. Degression of bcl-2 mRNA expression（P〈0.05） was detected by using RT-PC. Proliferation was inhibited by mistletoe alkali in SMMC-7721 cell line. Its mechanism was related to degression of bcl-2 mRNA expression.

关 键 词：槲寄生碱 BCL-2MRNA RT-PCR 人肝癌细胞SMMC-7721 

分 类 号：R931.7[医药卫生—生药学]