POU1 F1基因多态性与南方荷斯坦牛产奶量的相关性分析  

Correlation Between Polymorphism of POU1 F1 Gene and Milk Yield in Southern Chinese Holstein Cows

作　　者：谈成[1] 许惠[1] 侯明俸[1] 刘德武[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《中国奶牛》2010年第12期1-3,共3页China Dairy Cattle

摘　　要：采用PCR-RFLP技术研究了364头荷斯坦牛POU1 F1基因第二外显子的TaqⅠ酶切位点(e2-TaqⅠ)和第六外显子的HinfⅠ酶切位点(e6-HinfⅠ)多态性,并分析了两个位点多态性与泌乳量的关系。结果表明,实验群体中这两个多态位点均处于Hardy-Weinberg平衡状态,e2-TaqⅠ位点等位基因G/A的频率为0.2706/0.7294,e6-HinfⅠ位点等位基因G/A频率为0.3228/0.6772。性状关联结果表明,两个位点的基因型与第一、二胎泌乳量相关性不明显。PCR-RFLP method was conducted to detect single nucleotide polymorphism of exon 2 and exon 6 of POU1 F1 gene in 364 Southern Chinese Holstein Cows,and the correlation of polymorphisms of POU1 F1 gene and milk yield was analyzed.The results showed that the two polymorphism sites were at Hardy-Weinberg equilibrium.Moreover,the frequencies of allele G/A were 0.2706/0.7294,0.3228/0.6772 respectively.Correlation analysis showed that no significant associations were observed between the polymorphism of single site and milk yield traits.

关 键 词：奶牛 泌乳量 POU1 F1基因 PCR-RFLP 

分 类 号：S823.2[农业科学—畜牧学]