雪花莲外源凝集素基因的克隆及其多态性分析  

Cloning and Sequencing of Snowdrop Lectin cDNA and Polymorphism Analysis

作　　者：朱玉[1] 朱祯[1] 徐鸿林[1] 

机构地区：[1]中国科学院遗传研究所植物遗传操作实验室,北京100101

出　　处：《高技术通讯》1999年第9期36-41,共6页Chinese High Technology Letters

基　　金：863计划 ;国家自然科学基金 ;洛克菲勒 (RF97001# 581) 资助项目

摘　　要：从雪花莲（Ｇａｌａｎｔｈｕｓｎｉｖａｉｌｓ） 叶片中分离出了长度为４８４ｂｐ 的雪花莲外源凝集素ｃＤＮＡ，包含了完整的雪花莲外源凝集素编码序列。通过限制性酶切图谱及ＤＮＡ序列分析， 从中筛选出５ 种核苷酸且氨基酸序列均有差别的外源凝集素基因ＧＮＡ１１、ＧＮＡ１２、ＧＮＡ１３、ＧＮＡ１４和ＧＮＡ１５。将这５ 个克隆与６个已报道的序列进行比较， 发现其中ＧＮＡ１４ 与ＧＮＡ１５同源性最高，它们之间核苷酸序列及推导出的氨基酸序列的同源性分别为９８０％ 和９６２％ ， ＬＥＣＧＮＡ１ 与ＧＮＡ１１ 同源性最低， 它们之间核苷酸序列及推导出的氨基酸序列的同源性分别为７７３％ 和６５０％ 。The GNA cDNA was amplified from total snowdrop leaves RNA by RT PCR, and was cloned into EcoRV site of pBluescript KS(+). DNA sequence analysis indicates that the cloned 484 bp DNA fragment carry the entire GNA coding sequences. Five clones, GNA11, GNA12, GNA13, GNA14 and GNA15, were selected and compared with six published genes sequence. The highest homology was found between GNA14 and GNA15, with the similarities of 98 0% and 96 2% at the nucleotide level and at the deduced amino acid level, respectively. LECGNA1 and GNA11 showed the lowest homology of 77.3% and 65.0% for the nucleotide sequence and the amino acid sequence.

关 键 词：雪花莲 外源凝集素 多基因家族 RT-PCR DNA序列 

分 类 号：Q945.5[生物学—植物学]