鸡痘病毒282E_4株基因组末端非必需区的序列测定与分析  

Sequence Analysis of 7.3kb BamHI Fragment in the FPV 282 E 4 Strain

作　　者：金宁一[1] 司兴奎[1] 顾万钧[1] 郭志儒[1] 罗坤石 毅殷震 

机构地区：[1]解放军农牧大学军事兽医研究所,长春130062

出　　处：《高技术通讯》1999年第9期46-51,共6页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：将鸡痘病毒（ｆｏｗｌｐｏｘ ｖｉｒｕｓ， ＦＰＶ）２８２Ｅ４ 株７３ｋｂ 的Ｂａｍ ＨＩ片段经亚克隆后，获得ｐＵＦａ、ｐＫＳＦｂ、ｐＵＦｃ和ｐＵＦｄ 四个重组策粒。采用ＡＢＩ３７７ＤＮＡ测序仪荧光标记法对上述质粒进行了序列测定， 并应用ＤＮＡｓｉｓ Ｖ７０ 同ＧｅｎＢａｎｋ 中已知的ＦＰＶ序列和痘苗病毒（ｖａｃｃｉｎｉａ ｖｉｒｕｓ， ＶＶ） 的Ｃｏｐｅｎｈａｇｅｎ 株的全序列进行同源性比较。此外， 对所测序列的开放读码框架（ｏｐｅｎ ｒｅａｄｉｎｇ ｆｒａｍ ｅｓ，ＯＲＦ） 的氨基酸序列同上述ＶＶ株中所有ＯＲＦ的氨基酸序列进行了同源性比较，并对各ＯＲＦ编码蛋白的疏水性、等电点。A 7.3 kb BamHI fragment whthin the genome of Fowlpox virus(FPV) strain 282E4 was sequenced after subcloning into 4 plasmids pUFa, KsFb, pUFd and pUFc. With the help of DNAsis V7.0, open reading frames(ORFs) and the corresponding amino acid sequence encoded by each predicted oRF in the fragment were predicted, and some characters of the peptide encoded by each predicted ORF were analyzed. Homology between the fragment and vaccinia virus strain Copenhagen, and the FPV sequences collected in Genebank were analyzed as well.

关 键 词：鸡痘病毒 序列分析 开放读码框架 FPV ORF 

分 类 号：S852.65[农业科学—基础兽医学]