苯二氮卓受体在依托咪酯致小鼠学习记忆障碍中的作用  

Effects of benzodiazepine on amnestic effect induced by etomidate in mice

作　　者：黄河[1] 李冰[1] 韩丹[1] 马柯[1] 朱杨子[1] 武玉清[2] 

机构地区：[1]徐州医学院麻醉学院 [2]徐州医学院麻醉药理学教研室江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室

出　　处：《中国临床药理学与治疗学》2010年第10期1092-1095,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81070889);江苏省高校自然科学基金重大项目(10KJA320052);江苏省高等学校大学生实践创新训练计划项目(2010797)

摘　　要：目的:观察苯二氮卓受体拮抗剂氟马西尼(flumazenil,Flu)对依托咪酯(etomidate,Eto)所致遗忘作用的影响。方法:昆明种小鼠48只,随机分为4组:生理盐水+脂肪乳(intralipid)组(NS+Z组),生理盐水+依托咪酯组(NS+Eto组),氟马西尼+脂肪乳组(Flu+Z组),氟马西尼+依托咪酯组(Flu+Eto组)。训练前20min皮下注射氟马西尼(0.1mg/kg)或生理盐水(0.1mL/10g),训练前5min腹腔注射脂肪乳(0.12mL/10g)或依托咪酯(3mg/kg)。在跳台实验和避暗实验中观察氟马西尼对依托咪酯导致遗忘小鼠错误次数(errortimes,ET)、跳台潜伏期(step down latency,SDL)和步入潜伏期(step through latency,STL)的影响。结果:氟马西尼可减少依托咪酯所致遗忘小鼠的ET、延长SDL和STL。结论:苯二氮卓类(benzodiaz-epines,BDZ)受体可能是依托咪酯致小鼠遗忘作用的重要靶位。AIM：To observe the effects of flumazenil（Flu） on amnestic effect induced by etomidate in mice.METHODS：48 Kunming mice were randomly divided into 4 groups：NS＋intralipid（Z） Group,NS＋etomidate（Eto） Group,Flu＋Z Group,Flu＋Eto Group.All mice were given Flu（0.1 mg/kg）or NS（0.1 mL/10 g） by subcutaneous injection 20 min before training,then mice in all groups were administered intralipid（0.12 mL/10 g） or Eto（3 mg/kg） by intraperitoneal injection 5 min before training.All mice were then conducted to take step-down test and step-through test.The latency of step through and step down as well as the errortimes were observed to evaluated the effects of flumazenil on the amnestic effect induced by etomidate.RESULTS：Etomidate significantly increased error times and shortened the step down latency and step through latency of mouse.CONCLUSION：Benzodiazepines（BDZ） receptor may be an important target for the amnestic effect induced by etomidate.

关 键 词：氟马西尼 依托咪酯 学习记忆 跳台实验 避暗实验 

分 类 号：R614[医药卫生—麻醉学]