西罗莫司对小鼠树突状细胞CD_(86)、I-A^b和Toll样受体4表达的影响  

Effects of sirolimos on the expression of CD_(86),I-A^b and TLR4 on mice dendritic cells

作　　者：何秀娟[1] 张罕 李冬妹[3] 邱勇[4] 龙军[1] 安云庆[1] 胡永秀[5] 

机构地区：[1]首都医科大学免疫学系,北京100069 [2]振国肿瘤中西医结合医院,北京100176 [3]北京红十字血液中心,北京100088 [4]北京世纪坛医院检验科,北京100038 [5]首都医科大学基础医学实验教学中心,北京100069

出　　处：《中国临床药理学与治疗学》2010年第10期1133-1138,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：首都医科大学科研基金项目(2007ZR01)

摘　　要：目的:研究西罗莫司(SRL)对小鼠骨髓源树突状细胞(DC)发育成熟和Toll样受体4(TLR4)mRNA表达的影响。方法:(1)用细胞因子定向诱导C57BL/6小鼠骨髓细胞分化为DC,在相差显微镜和扫描电镜下动态观察DC在分化发育不同阶段的典型形态。(2)用SRL处理DC,通过流式细胞仪测定CD11c、CD86、MHC-Ⅱ类分子(I-Ab)的表达及在脂多糖(LPS)刺激后各分子表达的变化。(3)实时定量PCR法检测SRL处理DCTLR4mRNA表达水平。结果:(1)在相差显微镜和扫描电镜下可以看到DC在分化发育不同阶段的典型形态。(2)SRL抑制LPS刺激后DC表面CD86和I-Ab表达的上调。(3)与常规培养的DC相比,SRL组DCTLR4mRNA表达水平明显增高。结论:SRL处理的DC可抵抗LPS的促成熟作用。SRL促进DCTLR4mRNA表达。AIM：To observe the effects of sirolimos（SRL） on the maturation of murine bone marrow-derived dendritic cells（DC） and on the transcription of toll-like receptor 4（TLR4）.METHODS：（1） DCs derived from C57BL/6 murine bone marrow cells were induced by cytokines,and morphology development of DCs was observed by inverted microscope and scanning electron microscope.（2） The expression of CD11c,CD86 and MHC classⅡmolecule of DCs were assessed by flow cytometry after treatment with SRL,and the changes of these molecules after stimulation with lipopolysaccharide（LPS）.（3） The TLR4 mRNA transcription was detected by real-time PCR after treating with or without SRL.RESULTS：（1） Typical morphology of DCs can be observed during the different stages.（2） SRL inhibited the expression of CD86,I-Ab of DCs after stimulation with LPS.（3） The transcription of TLR4 mRNA was increased in SRL group.CONCLUSION：DCs treated with SRL can resist to promoting the maturation of DCs by LPS.The expression of TLR4 mRNA could be increased by SRL.

关 键 词：西罗莫司 树突状细胞 TOLL样受体4 CD86 I-Ab 

分 类 号：R965.2[医药卫生—药理学]