市售纯牛奶乳清E_2和P_4水平与季节变更的关系  

The relationship between the concentrations of estradiol and progesterone in commercial whole milk and seasons alternation

作　　者：马亚萍[1,2] 徐庄剑[1,2] 王勍[1,2] 胡瑜[3] 赵建伟[3] 张文杰[1,2] 王喜平[1,2] 

机构地区：[1]苏州大学第四临床医学院 [2]无锡市第四人民医院儿科,江苏无锡214062 [3]无锡市第四人民医院检验科,江苏无锡214062

出　　处：《扬州大学学报（农业与生命科学版）》2010年第3期25-29,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：苏州大学医学发展基金资助项目(2007-21)

摘　　要：全年等时距收集3个(A、B和C)品牌市售纯牛奶共72个批号,采用免疫化学发光分析法检测乳清总雌二醇(TE2)和孕酮(P4),研究其水平与季节变更的关系。结果表明:第1阶段(春分至初秋阶段)B和C品牌乳清P4水平均分别高于其第2阶段(初秋至次年春分阶段)(P<0.05);第2阶段A品牌乳清P4水平高于C品牌(P<0.05);第1阶段乳清P4高于其第2阶段(P=0.001),而这一阶段的TE2差异不显著(P>0.05)。A、B和C品牌的各批号间,第1阶段乳清TE2的中位数分别为168、180和180 pg.mL-1,P4分别为1.76、1.85和1.38 ng.mL-1;第2阶段乳清TE2的中位数分别为180、160和160 pg.mL-1,P4分别为1.55、0.95和0.76 ng.mL-1。第1阶段所有批号中乳清TE2和P4的中位数分别为176 pg.mL-1和1.57 ng.mL-1,第2阶段分别为164 pg.mL-1和1.13 ng.mL-1。全年所有批号乳清TE2和P4的中位数分别为172 pg.mL-1和1.46 ng.mL-1。表明市售纯牛奶同品牌以及品牌间乳清P4水平存在差异,且春分至初秋阶段较高;批号间乳清TE2水平也有一定波动。Seventy two samples from three brands（A,B and C） of commercial whole milk were investigated in a year.A sample in a lot number was collected from every brand once every half a month.Total estradiol（TE2） and progesterone（P4） in whey were assayed by immunochemiluminometric assays to observe the relationship between TE2 or P4 in commercial whole milk and seasons alternation.The results showed that： P4 concentrations in brand B and C at the first stage（from Spring Equinox to early Autumn） were respectively higher than those at the second stage（from early Autumn to Spring Equinox in the next year）（P0.05 in both）.At the second stage,P4 concentration in the brand A was higher than that in the brand C（P0.05）.P4 concentration for the all of the lot numbers of 3 brands at the first stage was higher than that at the second stage（P=0.001）,and no statistically significant difference in TE2 was found between the two stages.At the first stage,median for TE2 among lot numbers about brand A,B and C were respectively 168,180 and 180 pg·mL（-1）,and for P4 were respectively 1.76,1.85 and 1.38 ng·mL（-1）.At the second stage,median for TE2 among lot numbers about brand A,B and C were respectively 180,160 and 160 pg·mL（-1）,and for P4 were respectively 1.55,0.95 and 0.76 ng·mL（-1）.Median for TE2 and P4 in all lot numbers at the first stage were respectively 176 pg·mL（-1） and 1.57 ng·mL（-1）,and at the second stage were respectively 164 pg·mL（-1） and 1.13 ng·mL（-1）.Median for TE2 and P4 in all lot numbers in a year were respectively 172 pg·mL（-1） and 1.46 ng·mL（-1）.It was concluded that there were significant differences in P4 of milk whey among the same brands as well as different brands,and P4 of milk whey from Spring Equinox to early Autumn was higher than that in other period in a year,and there was also fluctuation in TE2 of milk whey among lot numbers.

关 键 词：牛奶 雌二醇 孕酮 免疫化学发光分析法 季节 

分 类 号：Q579.1[生物学—生物化学] TS252.2[轻工技术与工程—农产品加工及贮藏工程]