黄瓜绿斑驳花叶病毒的分子检测及云南分离物的序列分析  

Molecular detection of cucumber green mottle mosaic virus and sequence analyses of Yunnan isolates

作　　者：吴鑫[1,2] 丁元明 何月秋[2] 李旻 李克海 王绍君 周剑 

机构地区：[1]云南省出入境检验检疫局,云南昆明650228 [2]云南农业大学植物保护学院,云南昆明650224

出　　处：《扬州大学学报（农业与生命科学版）》2010年第3期75-80,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：农业部重大植物疫情检测和风险预警关键技术研究项目(2008IK264);云南省自然科学基金资助项目(2008CD198)

摘　　要：选取黄瓜绿斑驳花叶病毒（CGMMV）全基因组中MP-CP-3′UTR的保守序列（位于全基因组中第5692~6335位点之间）,设计1对特异性PCR检测引物,对云南省疑似带有CGMMV的病叶进行血清学检测和RT-PCR检测,并将目的片段的基因序列与GenBank中登录的CGMMV其他分离物相对应的序列进行比对和分析。结果表明：已报道的CGMMV主要分为4大类群,云南分离物属于第1类群;云南分离物与辽宁分离物亲缘关系最近,可能源于同一株系。Cucumber green mottle mosaic virus（CGMMV） was one member of genus Tobamovirus and it was quarantine pest issued by Chinese government.This study designed one pair of specific PCR primer based on MP-CP-3′UTR conserved sequences in CGMMV complete genome and detected leaf samples with serology and RT-PCR technology.Comparison of the aimed gene sequence of Yunnan isolates with other isolates logined in GenBank was conducted and analyzed.The sequence analyse result showed that： the reported CGMMV was divided into four populations mainly;Yunnan isolates belonged to I population;genetic relationship of Yunnan isolates was close to Liaoning,they may originated from one strain.

关 键 词：黄瓜绿斑驳花叶病毒 检疫性有害生物 RT-PCR检测 血清学检测 序列分析 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]