miR-106b转基因小鼠的建立  

Establishment of miR-106b Transgenic Mouse

作　　者：王海林[1] 全雄志[1] 董伟[1] 宗园媛[1] 刘嘉琳[1] 秦川[1] 

机构地区：[1]中国医学科学院实验动物研究所国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国生物工程杂志》2010年第12期1-4,共4页China Biotechnology

基　　金：国家"十一五"新药专向支持(2009ZX09501-026);中央级公益性科研院所基本科研业务费专向基金(DWS200814)资助项目

摘　　要：目的:建立miR-106b转基因小鼠模型,探讨其在阿尔茨海默病(Alzheimer’s d isease,AD)发病中的作用。方法:构建miR-106b表达载体,显微注射法建立miR-106b转基因小鼠。PCR鉴定转基因小鼠的基因型,real time RT-PCR检测m iR-106b转基因小鼠脑组织中miR-106b的表达情况,W estern blot检测miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达。结果:构建了高表达m iR-106b转基因小鼠;与对照相比,miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达升高。结论:miR-106b转基因小鼠的建立为研究该microRNA在AD发病中的作用提供了工具。Objective：To establish a miR-106b transgenic mouse and investigate its effect on the development of Alzheimer＇s disease（AD）.Methods：The expression vector of miR-106b was constructed.The transgenic mouse was produced by microinjection and genotype was detected by PCR.The expression levels of miR-106b were detected by real time RT-PCR.The protein levels of TGFBR2 were detected by Western blot.Results：Five founders of miR-106b transgenic mice were established and one high-level expression line was identified.Compared with the wild type mice,the expression of TGFBR2 was increased in miR-106b transgenic mice.Conclusion：miR-106b transgenic mouse has been established and it can be used to investigate the function of miR-106b on the development of Alzheimer＇s disease.

关 键 词：miR-106b 阿尔茨海默病 TGFBR2 小鼠 转基因 脑 

分 类 号：Q789[生物学—分子生物学]